Anticorps Polyclonal de lapin anti-RPH3AL

RPH3AL Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 15297-1-AP

Synonymes

No C2 domains protein, NOC2, Rab effector Noc2, Rabphilin 3A like protein, RPH3AL

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Galerie de données de validation

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  WB analysis of human liver using 15297-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15297-1-AP (RPH3AL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 15297-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 15297-1-AP (RPH3AL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 15297-1-AP

  IP result of anti-RPH3AL (IP:15297-1-AP, 4ug; Detection:15297-1-AP 1:500) with HepG2 cells lysate 2400ug.

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Applications testées

• Résultats positifs en WB: tissu hépatique humain, cellules L02
• Résultats positifs en IP: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

15297-1-AP cible RPH3AL dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RPH3AL Protéine recombinante Ag7552
• Nom complet: rabphilin 3A-like (without C2 domains)
• Masse moléculaire calculée: 34 kDa
• Poids moléculaire observé: 38 kDa
• Numéro d’acquisition GenBank: BC005153
• Symbole du gène: RPH3AL
• Identification du gène (NCBI): 9501
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Rabphilin-3A-like (RPH3AL), also known as Noc2, is a Rab effector protein with a molecular weight of 38 kDa. It is expressed abundantly in endocrine cells but at low levels in exocrine tissues, playing a direct regulatory role in calcium-ion-dependent exocytosis in both endocrine and exocrine cells, and also having a key role in INS secretion by pancreatic cells. RPH3AL functions as a potential RAB3B effector protein in epithelial cells, and RPH3AL gene is likely a tumor suppressor.

Publications

Species	Application	Title
human	WB	J Cell Sci; Rab2a and Rab27a cooperatively regulate transition from granule maturation to exocytosis through the dual effector Noc2.; Authors - Kohichi Matsunaga
human	WB	Clin Chim Acta; Detection of autoantibodies against Rabphilin-3A-like protein as a potential biomarker in patient's sera of colorectal cancer.; Authors - Jinn-Shiun Chen

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