Anticorps Polyclonal de lapin anti-NOL12

• Phare
• Validé par KD/KO

NOL12 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 15456-1-AP

Synonymes

Nucleolar protein 12, Nop25

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Galerie de données de validation

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  WB analysis using 15456-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15456-1-AP (NOL12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15456-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15456-1-AP (NOL12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 15456-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15456-1-AP (NOL12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15456-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15456-1-AP (NOL12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15456-1-AP

  IP result of anti-NOL12 (IP:15456-1-AP, 4ug; Detection:15456-1-AP 1:300) with HeLa cells lysate 4000ug.

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  IF Staining of HepG2 using 15456-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NOL12 antibody (15456-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules Raji, cellules SH-SY5Y
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

15456-1-AP cible NOL12 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NOL12 Protéine recombinante Ag7723
• Nom complet: nucleolar protein 12
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC002808
• Symbole du gène: NOL12
• Identification du gène (NCBI): 79159
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Nucleolar protein 12 (NOL12), a multifunctional RNA-binding protein, has been found to be related to genomic stability, DNA damage repair, and apoptosis. High expression of NOL12 is associated with worse reduced overall survival (OS), high pathological grade, node metastasis, and advanced clinical stage in patients with HCC (PMID: 35693698). Knockdown of NOL12 impairs cellular proliferation by inducing a G1/S cell cycle arrest, but does so outside of a nucleolar stress response and in a p53-independent manner (PMID: 29069457).

Publications

Species	Application	Title
human	WB	Nature; Global landscape of HIV-human protein complexes.; Authors - Stefanie Jäger
human,rat	IHC,WB,IF,CoIP	Cell Death Discov; Protein expression of nucleolar protein 12 in the retina and its implication in protection of retina from UV irradiation damage; Authors - Jingtao Liu ; KD Validated

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