Anticorps Polyclonal de lapin anti-NOTCH3

• Phare
• Validé par KD/KO

NOTCH3 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

45

N° de cat : 55114-1-AP

Synonymes

CADASIL, CASIL, Notch 3, Notch homolog 3 (Drosophila), NOTCH3

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Galerie de données de validation

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  WB analysis using 55114-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55114-1-AP (NOTCH3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 55114-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55114-1-AP (NOTCH3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of U2OS using 55114-1-AP

  U2OS cells were subjected to SDS PAGE followed by western blot with 55114-1-AP (NOTCH3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 55114-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 55114-1-AP (NOTCH3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 55114-1-AP

  IP result of anti-NOTCH3(IP:55114-1-AP, 4ug; Detection:55114-1-AP 1:800) with HeLa cells lysate 2000 ug.

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  IHC staining of human lung cancer using 55114-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55114-1-AP (NOTCH3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 55114-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55114-1-AP (NOTCH3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 55114-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55114-1-AP (NOTCH3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 55114-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55114-1-AP (NOTCH3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 55114-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55114-1-AP (NOTCH3 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 55114-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55114-1-AP (NOTCH3 Antibody) at dilution of 1:200 (under 40x lens).

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  FC experiment of Jurkat using 55114-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human NOTCH3 (55114-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A2780, cellules Caco-2, cellules COLO 320, cellules HeLa, cellules K-562, cellules U2OS
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer du sein humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 36 publications below
• IHC: See 13 publications below
• IF: See 4 publications below

Informations sur le produit

55114-1-AP cible NOTCH3 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: Notch homolog 3 (Drosophila)
• Masse moléculaire calculée: 244 kDa
• Poids moléculaire observé: 250-260 kDa, 100 kDa
• Numéro d’acquisition GenBank: NM_000435
• Symbole du gène: NOTCH3
• Identification du gène (NCBI): 4854
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NOTCH3 belongs to the NOTCH family. It functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. NOTCH3 affects the implementation of differentiation, proliferation and apoptotic programs. Defects in NOTCH3 are the cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). The antibody is specific to NOTCH3. Notch3 is a single-pass type I membrane protein exposed at the cell surface as a heterodimer of 2321 amino acids. It is synthesized as a precursor with a molecular weight of ~280 kDa (Notch3 full-length), which is cleaved (S1 cleavage) into a 210 kDa extracellular fragment and a 97 kDa intracellular fragment (PMID: 21702048).

Publications

Species	Application	Title
mouse	WB	Cell Metab; Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells.; Authors - Xiuying Zhong
human,mouse,rat	WB,IF	Sci Transl Med; JAGGED-NOTCH3 signaling in vascular remodeling in pulmonary arterial hypertension.; Authors - Yu Zhang
mouse	WB	Mol Ther; METTL3-mediated m6A modification of TIMP2 mRNA promotes podocyte injury in diabetic nephropathy.; Authors - Ling Jiang
rat	WB	Biomaterials; Laminin-521 promotes quiescence in isolated stellate cells from rat liver.; Authors - Friederike Rohn
mouse	WB	Cancer Res; Manic fringe promotes a claudin-low breast cancer phenotype through notch-mediated PIK3CG induction.; Authors - Shubing Zhang
human	WB	Oncogene; Notch3 promotes prostate cancer-induced bone lesion development via MMP-3.; Authors - Sourik S Ganguly ; KD Validated

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