Anticorps Polyclonal de lapin anti-NOVA2

• Phare
• Validé par KD/KO

NOVA2 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 55002-1-AP

Synonymes

ANOVA, NOVA2, NOVA3, RNA binding protein Nova 2

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Galerie de données de validation

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  WB analysis of mouse brain using 55002-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55002-1-AP (NOVA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 55002-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55002-1-AP (NOVA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 55002-1-AP

  IP result of anti-NOVA2 (IP:55002-1-AP, 4ug; Detection:55002-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en IP: tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below

Informations sur le produit

55002-1-AP cible NOVA2 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: neuro-oncological ventral antigen 2
• Masse moléculaire calculée: 49 kDa
• Poids moléculaire observé: 50-55 kDa, 65-70 kDa
• Numéro d’acquisition GenBank: NM_002516
• Symbole du gène: NOVA2
• Identification du gène (NCBI): 4858
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NOVA2 also named as ANOVA and NOVA3. It regulates RNA splicing or metabolism in a specific subset of developing neurons. NOVA2 binds single strand RNA. The antibody is specific to NOVA2. By the alternative splicing, NOVA2 can be detected as 50-55 and 70-80 kDa (PMID:26446569, PMID:9789075).

Publications

Species	Application	Title
human	WB	Acta Neuropathol; Aberrant NOVA1 function disrupts alternative splicing in early stages of amyotrophic lateral sclerosis.; Authors - Florian Krach
mouse	WB	Aging (Albany NY); PMID: 32271715; Authors - Dongdong Chai ; KD Validated
mouse	WB	Nutrients; Nova1 or Bim Deficiency in Pancreatic β-Cells Does Not Alter Multiple Low-Dose Streptozotocin-Induced Diabetes and Diet-Induced Obesity in Mice; Authors - Manoja K Brahma
human	WB	Hepatol Commun; METTL14 reverses liver fibrosis by inhibiting NOVA2 through an m6A-YTHDF2-dependent mechanism; Authors - Xiaoxue Hou
human	WB	medRxiv; Spatial proteo-transcriptomic profiling reveals the molecular landscape of borderline ovarian tumors and their invasive progression; Authors - Lisa Schweizer
human	WB	Neuron; Inhibition of RNA splicing triggers CHMP7 nuclear entry, impacting TDP-43 function and leading to the onset of ALS cellular phenotypes; Authors - Norah Al-Azzam

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