Anticorps Polyclonal de lapin anti-BNP

BNP Polyclonal Antibody for IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 13299-1-AP

Synonymes

BNP, BNP(1 32), Natriuretic peptides B, NPPB

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Galerie de données de validation

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  IHC staining of mouse brain using 13299-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13299-1-AP (BNP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 13299-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13299-1-AP (BNP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 13299-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 13299-1-AP (BNP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 13299-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 13299-1-AP (BNP Antibody) at dilution of 1:50.

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  IHC staining of human brain using 13299-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 13299-1-AP (BNP Antibody) at dilution of 1:50.

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  IHC staining of human heart using 13299-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 13299-1-AP (BNP Antibody) at dilution of 1:50.

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  IHC staining of human heart using 13299-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 13299-1-AP (BNP Antibody) at dilution of 1:50.

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  IF Staining of rat brain using 13299-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using BNP antibody (13299-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 13299-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using BNP antibody (13299-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en IHC: tissu cérébral de souris, tissu cardiaque humain, tissu cérébral de rat, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu cérébral de rat, cellules HepG2

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 2 publications below

Informations sur le produit

13299-1-AP cible BNP dans les applications de IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BNP Protéine recombinante Ag4039
• Nom complet: natriuretic peptide precursor B
• Masse moléculaire calculée: 134 aa, 15 kDa
• Numéro d’acquisition GenBank: BC025785
• Symbole du gène: NPPB
• Identification du gène (NCBI): 4879
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NPPB, also known as B-type natriuretic peptide (BNP), is a cardiac hormone that is secreted mainly in the ventricles in response to increased wall stress. The protein undergoes two cleavage events, one within the cell and a second after secretion into the blood. The protein's biological actions include natriuresis, diuresis, vasorelaxation, inhibition of renin and aldosterone secretion, and a key role in cardiovascular homeostasis. BNP is a marker of systolic and diastolic dysfunction and a strong predictor of mortality in heart failure patients.

Publications

Species	Application	Title
rat		Front Cardiovasc Med; Effects of Excessive Activation of N-methyl-D-aspartic Acid Receptors in Neonatal Cardiac Mitochondrial Dysfunction Induced by Intrauterine Hypoxia.; Authors - Yang Liu
		Pharm Biol; Epigallocatechin gallate (EGCG) attenuates myocardial hypertrophy and fibrosis induced by transverse aortic constriction via inhibiting the Akt/mTOR pathway.; Authors - Yue Cui
		Int Heart J; Phenotype and Functional Analyses in a Transgenic Mouse Model of Left Ventricular Noncompaction Caused by a DTNA Mutation.; Authors - Qing Cao
rat		Cell Regen; Fabrication of SA/Gel/C scaffold with 3D bioprinting to generate micro-nano porosity structure for skin wound healing: a detailed animal in vivo study.; Authors - Changmei Niu
	IHC	Front Pharmacol; Administration of USP7 inhibitor P22077 inhibited cardiac hypertrophy and remodeling in Ang II-induced hypertensive mice; Authors - Yu-Hui Gu
rat		Mol Med Rep; G9a inhibition promotes the formation of pacemaker-like cells by reducing the enrichment of H3K9me2 in the HCN4 promoter region; Authors - Pei Xu