Anticorps Polyclonal de lapin anti-Neuropeptide Y

Neuropeptide Y Polyclonal Antibody for IHC, IF-P, IF-Fro, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, IF-Fro, ELISA

Conjugaison

Non conjugué

Publications(14)

Avis (1)

N° de cat : 12833-1-AP

Synonymes

NPY, C-flanking peptide of NPY, CPON, Neuropeptide tyrosine, Pro-neuropeptide Y

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Galerie de données de validation

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  IHC staining of human brain using 12833-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12833-1-AP (Neuropeptide Y antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human brain using 12833-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12833-1-AP (Neuropeptide Y antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human pancreas cancer using 12833-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12833-1-AP (Neuropeptide Y antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas cancer using 12833-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12833-1-AP (Neuropeptide Y antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of rat brain using 12833-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12833-1-AP (Neuropeptide Y antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of rat brain using 12833-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12833-1-AP (Neuropeptide Y antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of mouse brain using 12833-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12833-1-AP (Neuropeptide Y antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12833-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12833-1-AP (Neuropeptide Y antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 12833-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12833-1-AP (Neuropeptide Y antibody) at dilution of 1:50 (under 25x lens).

• 

  IF Staining of mouse brain using 12833-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Neuropeptide Y antibody (12833-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 12833-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Neuropeptide Y antibody (12833-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse eye using 12833-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using Neuropeptide Y antibody (12833-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse eye using 12833-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using Neuropeptide Y antibody (12833-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of rat brain using 12833-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat brain tissue using Neuropeptide Y antibody (12833-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en IHC: tissu cérébral humain, tissu cérébral de rat, tissu cérébral de souris, tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris, tissu oculaire de souris
• Résultats positifs en IF-Fro: tissu cérébral de rat,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 7 publications below
• IF: See 4 publications below

Informations sur le produit

12833-1-AP cible Neuropeptide Y dans les applications de WB, IHC, IF-P, IF-Fro, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Neuropeptide Y Protéine recombinante Ag3568
• Nom complet: neuropeptide Y
• Masse moléculaire calculée: 97 aa, 11 kDa
• Numéro d’acquisition GenBank: BC029497
• Symbole du gène: Neuropeptide Y
• Identification du gène (NCBI): 4852
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Neuropeptide Y (NPY) is one of the most abundant peptides in the mammalian central nervous system (CNS). NPY has been linked to several physiological and pathological functions, such as feeding behaviour, memory processing, pain, anxiety, cell proliferation and many other processes in the central and peripheral nervous systems. The neuropeptide functions through G protein-coupled receptors to inhibit adenylyl cyclase, activate mitogen-activated protein kinase (MAPK), regulate intracellular calcium levels, and activate potassium channels. In addition, NPY was suggested as a potential neuroprotective agent in Alzheimer's disease by counteracting the toxic effect of β-amyloid in an in vitro model.

Publications

Species	Application	Title
human,mouse	IHC,IF	Nat Aging; Single-cell and spatial RNA sequencing identify divergent microenvironments and progression signatures in early- versus late-onset prostate cancer; Authors - Yifei Cheng
rat	IHC	ACS Appl Mater Interfaces; Lithium-Doped Titanium Dioxide-Based Multilayer Hierarchical Structure for Accelerating Nerve-Induced Bone Regeneration; Authors - Qianqian Zhang
mouse	WB	Free Radic Biol Med; Inhibition of neuronal nitric oxide synthase protects against hippocampal neuronal injuries by increasing neuropeptide Y expression in temporal lobe epilepsy mice.; Authors - Yuanyuan Yao
human	IHC	J Orthop Translat; Suprascapular nerve injury affects rotator cuff healing: A paired controlled study in a rat model.; Authors - Yucheng Sun
rat	IF	Front Behav Neurosci; Heterogeneous GAD65 Expression in Subtypes of GABAergic Neurons Across Layers of the Cerebral Cortex and Hippocampus.; Authors - Yuki Kajita
		Acta Neurobiol Exp (Wars); Aberrant changes of somatostatin and neuropeptide Y in brain of a genetic rat model for epilepsy: tremor rat.; Authors - Xiaoxue Xu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Reyes (Verified Customer) (04-12-2024)

NPY (green) worked nicely marking a subpopulation of interneurons in human brain FFPE cortex tissue.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

Neuropeptide Y Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:12833-1-AP)

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