Anticorps Polyclonal de lapin anti-NR1H3

• Phare
• Validé par KD/KO

NR1H3 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(100)

Avis (1)

N° de cat : 14351-1-AP

Synonymes

Liver X receptor alpha, LXR a, LXRA, LXRα, Oxysterols receptor LXR alpha

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Galerie de données de validation

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  WB analysis of HL-60 using 14351-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 14351-1-AP (NR1H3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14351-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14351-1-AP (NR1H3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat liver using 14351-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 14351-1-AP (NR1H3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 14351-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 14351-1-AP (NR1H3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules COLO 320, cellules THP-1, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IHC: tissu hépatique de rat, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 10 publications below
• WB: See 96 publications below
• IHC: See 6 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Informations sur le produit

14351-1-AP cible NR1H3 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, Lapin, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NR1H3 Protéine recombinante Ag5697
• Nom complet: nuclear receptor subfamily 1, group H, member 3
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC041172
• Symbole du gène: NR1H3
• Identification du gène (NCBI): 10062
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NR1H3, also known as LXRA, is a liver X receptors, which form a subfamily of the nuclear receptor superfamily and are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. It's also a ligand-activated LXRs and forms heterodimers with retinoid X receptor, then participates in regulating expression of target genes containing LXR response elements. NR1H3 exists some isoforms with MV 51 kDa and 43-45 kDa.

Publications

Species	Application	Title
	WB	Nat Commun; p21-activated kinase 4 suppresses fatty acid β-oxidation and ketogenesis by phosphorylating NCoR1; Authors - Min Yan Shi
human	WB	Br J Pharmacol; Functional interplay between LXR and AMPKα inhibits atherosclerosis in apoE deficient mice - A new anti-atherogenic strategy.; Authors - Chuanrui Ma
mouse	WB	Theranostics; Encapsulation of LXR ligand by D-Nap-GFFY hydrogel enhances anti-tumorigenic actions of LXR and removes LXR-induced lipogenesis.; Authors - Ke Feng
human	WB	EMBO Rep; Lin28 enhances de novo fatty acid synthesis to promote cancer progression via SREBP-1.; Authors - Yang Zhang
mouse	WB,IF	Food Res Int; Cabernet sauvignon dry red wine ameliorates atherosclerosis in mice by regulating inflammation and endothelial function, activating AMPK phosphorylation, and modulating gut microbiota; Authors - Xinlong Cheng
mouse,human	WB	Phytomedicine; Rice bran active peptide (RBAP) inhibited macrophage differentiation to foam cell and atherosclerosis in mice via regulating cholesterol efflux; Authors - Jianfei Mu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

K (Verified Customer) (06-10-2021)

works good in rat and human cells form western blotting.

• Applications: Western Blot
• Primary Antibody Dilution: 1;1000
• Cell Tissue Type: rat and human liver cells

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