Anticorps Polyclonal de lapin anti-NR1H4

• Phare
• Validé par KD/KO

NR1H4 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (3)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(85)

Avis (1)

N° de cat : 25055-1-AP

Synonymes

BAR, Bile acid receptor, Farnesoid X activated receptor, Farnesoid X-activated receptor, Farnesol receptor HRR-1

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Galerie de données de validation

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  WB analysis of HepG2 using 25055-1-AP

  WB result of NR1H4 antibody (25055-1-AP; 1:4000; room temperature for 1.5 hours) with wild-type and NR1H4 knockout HepG2 cells.

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  WB analysis using 25055-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25055-1-AP (NR1H4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of rat liver using 25055-1-AP

  IP result of anti-NR1H4 (IP:25055-1-AP, 4ug; Detection:25055-1-AP 1:2000) with rat liver tissue lysate 2600 ug.

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  IHC staining of human kidney using 25055-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25055-1-AP (NR1H4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 25055-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25055-1-AP (NR1H4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules MCF-7, cellules MDA-MB-231
• Résultats positifs en IP: tissu hépatique de rat,
• Résultats positifs en IHC: tissu rénal humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 7 publications below
• WB: See 71 publications below
• IHC: See 16 publications below
• IF: See 19 publications below
• IP: See 2 publications below
• CoIP: See 2 publications below

Informations sur le produit

25055-1-AP cible NR1H4 dans les applications de WB, IHC, IF, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NR1H4 Protéine recombinante Ag21878
• Nom complet: nuclear receptor subfamily 1, group H, member 4
• Masse moléculaire calculée: 486 aa, 56 kDa
• Poids moléculaire observé: 48-56 kDa
• Numéro d’acquisition GenBank: BC130573
• Symbole du gène: NR1H4
• Identification du gène (NCBI): 9971
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Nuclear Receptor subfamily 1, group H, member 4 (NR1H4, also known as FXR) is a receptor for bile acids and has an important role in regulating energy metabolism in liver, muscle and adipose tissues in humans and animals. NR1H4 is highly expressed in liver and has a role in lipid metabolism, whereas none of the other protein-coding genes in the region has known biological links with cholesterol, further supporting a role for NR1H4 in regulation of cholesterol levels. NR1H4 have five isoforms, each isoform has a different expressional level and transcriptional activity depending on its location within specific tissues. (PMID: 14733360, PMID: 30787420)

Publications

Species	Application	Title
mouse	WB	Cell Host Microbe; Dietary fiber alleviates alcoholic liver injury via Bacteroides acidifaciens and subsequent ammonia detoxification; Authors - Haiyuan Shen ; KD Validated
human	CoIP	Cell Host Microbe; A gut microbiota-bile acid axis promotes intestinal homeostasis upon aspirin-mediated damage; Authors - Ting Li
mouse	WB,IF	Sci Total Environ; Bisphenol A induced hepatic steatosis by disturbing bile acid metabolism and FXR/TGR5 signaling pathways via remodeling the gut microbiota in CD-1 mice; Authors - Ting Hong
rat	WB,IF	Phytomedicine; Wen-Shen-Jian-Pi-Hua-Tan decoction protects against early obesity-related glomerulopathy by improving renal bile acid composition and suppressing lipogenesis, inflammation, and fibrosis; Authors - Daofei Song
rat	WB	Phytomedicine; Jiang-Tang-San-Huang pill alleviates type 2 diabetes mellitus through modulating the gut microbiota and bile acids metabolism; Authors - Dina Tawulie
mouse,human	WB,IHC	Biomater Sci; Mesenchymal stem cell-derived exosome mitigates colitis via the modulation of the gut metagenomics-metabolomics-farnesoid X receptor axis; Authors - Dickson Kofi Wiredu Ocansey

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Bryce (Verified Customer) (04-16-2021)

Antibody did not work for mouse kidney homogenates. There was no observable difference between kidneys from wild type and knockout mice. Genotypes of blotted samples were confirmed by RT-qPCR.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse kidney

NR1H4 Antibody Western Blot validation (1:1000 dilution) in mouse kidney (Cat no:25055-1-AP)

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