Anticorps Polyclonal de lapin anti-NR5A2

• Phare
• Validé par KD/KO

NR5A2 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 22460-1-AP

Synonymes

B1 binding factor, B1F, B1F2, CPF, CYP7A promoter binding factor, FTF, FTZ F1, FTZ F1beta, hB1F, hB1F 2, Liver receptor homolog 1, LRH 1, NR5A2

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Galerie de données de validation

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  WB analysis using 22460-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22460-1-AP (NR5A2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 22460-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 22460-1-AP (NR5A2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat testis using 22460-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 22460-1-AP (NR5A2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules BxPC-3, tissu hépatique de souris, tissu ovarien de souris, tissu pancréatique de souris, tissu testiculaire de rat

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

22460-1-AP cible NR5A2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NR5A2 Protéine recombinante Ag18001
• Nom complet: nuclear receptor subfamily 5, group A, member 2
• Masse moléculaire calculée: 541 aa, 61 kDa
• Poids moléculaire observé: 61-67 kDa
• Numéro d’acquisition GenBank: BC118652
• Symbole du gène: NR5A2
• Identification du gène (NCBI): 2494
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NR5A2 belongs to the nuclear hormone receptor family NR5 subfamily.It binds to the sequence element 5'-AACGACCGACCTTGAG-3' of the enhancer II of hepatitis B virus genes, a critical cis-element of their expression and regulation.NR5A2 may be responsible for the liver-specific activity of enhancer II, probably in combination with other hepatocyte transcription factors. Key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver. It may also contribute to the regulation of pancreas-specific genes and play important roles in embryonic development. LRH-1 is phosphorylated to display its normal function, including its role in the intriguing potential responses to phospholipid or other ligands. The hinge domain serine residues at 238 and 243 are required for effective phosphorylation, both in vitro and in cells (16439367).NR5A1 is abundantly expressed in pancreas, less in liver, very low levels in heart and lung. Expressed in hepG2. Isoform 1 and isoform 2 seem to be present in fetal and adult liver and hepG2 cells.The observed molecular weight of 65-70 kDa is the posphorylation of NR5A2 protein.

Publications

Species	Application	Title
mouse	WB	Aging (Albany NY); NR5A2 as a potential target for exercise to improve metabolic syndrome; Authors - Lingxiu Meng
human,mouse	WB,IHC,IF	Cell Prolif; LRH-1 activation alleviates diabetes-induced podocyte injury by promoting GLS2-mediated glutaminolysis; Authors - Jijia Hu
human	WB	Mol Ther Nucleic Acids; MicroRNA-3613-5p Promotes Lung Adenocarcinoma Cell Proliferation through a RELA and AKT/MAPK Positive Feedback Loop.; Authors - Tao He ; KD Validated
mouse	WB	Pharmacol Res; Geniposide reduces cholesterol accumulation and increases its excretion by regulating the FXR-mediated liver-gut crosstalk of bile acids.; Authors - Jinxin Liu
human	WB,IHC	Front Oncol; NR5A2 Is One of 12 Transcription Factors Predicting Prognosis in HNSCC and Regulates Cancer Cell Proliferation in a p53-Dependent Manner.; Authors - Kun Zhang ; KD Validated
human	WB, IHC	Cell Death Discov; NR5A2 transcriptional activation by BRD4 promotes pancreatic cancer progression by upregulating GDF15.; Authors - Feng Guo