Anticorps Polyclonal de lapin anti-GCNF

• Phare
• Validé par KD/KO

GCNF Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 12712-1-AP

Synonymes

GCNF, GCNF1, Germ cell nuclear factor, hGCNF, hRTR, NR61, NR6A1, RTR

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Galerie de données de validation

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  WB analysis of PC-3 using 12712-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12712-1-AP (GCNF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 12712-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12712-1-AP (GCNF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 12712-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12712-1-AP (GCNF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 12712-1-AP

  IP Result of anti-GCNF (IP:12712-1-AP, 4ug; Detection:12712-1-AP 1:800) with mouse testis tissue lysate 4000ug.

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  IHC staining of human testis using 12712-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12712-1-AP (GCNF antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 12712-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12712-1-AP (GCNF antibody at dilution of 1:50 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules PC-3, cellules K-562, tissu testiculaire de souris
• Résultats positifs en IP: tissu testiculaire de souris
• Résultats positifs en IHC: tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

12712-1-AP cible GCNF dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GCNF Protéine recombinante Ag3395
• Nom complet: nuclear receptor subfamily 6, group A, member 1
• Masse moléculaire calculée: 475 aa, 54 kDa
• Poids moléculaire observé: 54 kDa
• Numéro d’acquisition GenBank: BC030600
• Symbole du gène: NR6A1
• Identification du gène (NCBI): 2649
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NR6A1 (Nuclear Receptor Subfamily 6 Group A member), also known as GCNF (Germ Cell Nuclear Factor), is an orphan member of the nuclear receptor gene superfamily. It has been shown to be expressed in the nervous system during development and during specific stages in maturing germ cells of the ovary and testis in the adult, and has probable roles in gametogenesis, neurogenesis, and normal embryonic development during gastrulation. Inactivation of GCNF in mouse results in abnormal posterior development, impaired midbrain development, insufficient closure of the neural tube, and eventual embryonic death. GCNF has been shown to be a repressor of OCT-4 and plays a critical role in the control of gene expression during embryogenesis and spermatogenesis. This antibody specifically reacts with the 55kd NR6A1 protein.

Publications

Species	Application	Title
mouse	WB,IF	Neuropharmacology; Hippocampal NR6A1 impairs CREB-BDNF signaling and leads to the development of depression-like behaviors in mice.; Authors - Pingping Tan ; KD Validated
mouse	IHC	Cancer Med; microRNA-196a-5p inhibits testicular germ cell tumor progression via NR6A1/E-cadherin axis.; Authors - Xiaowen Liu ; KD Validated
human	WB	J Clin Transl Hepatol; Establishment of a Prognostic Model for Hepatocellular Carcinoma Based on Bioinformatics and the Role of NR6A1 in the Progression of HCC; Authors - Zhong-Hua Lin