Anticorps Polyclonal de lapin anti-NRBP2

• Phare
• Validé par KD/KO

NRBP2 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 21549-1-AP

Synonymes

NRBP2, pp9320, TRG 16, TRG16

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Galerie de données de validation

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  WB analysis of mouse kidney using 21549-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21549-1-AP (NRBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 21549-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 21549-1-AP (NRBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 21549-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 21549-1-AP (NRBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 21549-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21549-1-AP (NRBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21549-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21549-1-AP (NRBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 21549-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21549-1-AP (NRBP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 21549-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 21549-1-AP (NRBP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 21549-1-AP

  IP Result of anti-NRBP2 (IP:21549-1-AP, 4ug; Detection:21549-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human kidney using 21549-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21549-1-AP (NRBP2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 21549-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21549-1-AP (NRBP2 Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, cellules A549, cellules HeLa, tissu cérébral de souris, tissu placentaire humain, tissu testiculaire humain
• Résultats positifs en IP: tissu rénal de souris
• Résultats positifs en IHC: tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 2 publications below

Informations sur le produit

21549-1-AP cible NRBP2 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NRBP2 Protéine recombinante Ag16215
• Nom complet: nuclear receptor binding protein 2
• Masse moléculaire calculée: 501 aa, 58 kDa
• Poids moléculaire observé: 33 kDa, 58 kDa
• Numéro d’acquisition GenBank: BC113873
• Symbole du gène: NRBP2
• Identification du gène (NCBI): 340371
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Nuclear receptor binding protein 2(NRBP2), also named as TRG16, belongs to the Ser/Thr protein kinase family. NRBP2 can regulate apoptosis of neural progenitor cells during their differentiation. Expression of NRBP2 was up-regulated following differentiation in rodent neural stem/progenitor cells. It has 4 isoforms with MW of 58, 33, 36 and 14 kDa. Endogenous NRBP2 in HEK293 cells has an apparent molecular mass of 55-60 kD by SDS-PAGE.

Publications

Species	Application	Title
human	WB,IHC	Front Oncol; NRBP2 Functions as a Tumor Suppressor and Inhibits Epithelial-to-Mesenchymal Transition in Breast Cancer.; Authors - Zhiyu Li ; KD Validated
human	WB	Ann Transl Med; PSMD12 promotes glioma progression by upregulating the expression of Nrf2.; Authors - Zhongyong Wang
human,mouse	WB,IHC	Mol Cell Biochem; TRG16, targeted by miR-765, inhibits breast cancer stem cell-like properties via regulating the NF-κB pathway.; Authors - Feng Chi ; KD Validated
human	WB	Cancers (Basel); Nuclear Receptor Binding Protein 2 Is Downregulated in Medulloblastoma, and Reduces Tumor Cell Survival upon Overexpression.; Authors - Anqi Xiong

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