Anticorps Polyclonal de lapin anti-NRF1/nuclear respiratory factor 1

• Phare
• Validé par KD/KO

NRF1/nuclear respiratory factor 1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (5)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(99)

N° de cat : 12482-1-AP

Synonymes

NRF1, nuclear respiratory factor 1, ALPHA PAL, Alpha palindromic-binding protein, Alpha-pal

---

Galerie de données de validation

• 

  WB analysis using 12482-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12482-1-AP (NRF1/nuclear respiratory factor 1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human lung using 12482-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12482-1-AP (NRF1/nuclear respiratory factor 1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skeletal muscle using 12482-1-AP

  IP result of anti-NRF1/nuclear respiratory factor 1 (IP:12482-1-AP, 4ug; Detection:12482-1-AP 1:300) with mouse skeletal muscle tissue lysate 3000ug.

• 

  IHC staining of human stomach using 12482-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 12482-1-AP (NRF1/nuclear respiratory factor 1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293T, tissu pulmonaire humain
• Résultats positifs en IHC: tissu d'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 96 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

12482-1-AP cible NRF1/nuclear respiratory factor 1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Chèvre, Humain, poisson-zèbre, porc, poulet, souris, duck
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NRF1/nuclear respiratory factor 1 Protéine recombinante Ag3172
• Nom complet: nuclear respiratory factor 1
• Masse moléculaire calculée: 522 aa, 56 kDa
• Poids moléculaire observé: 67 kDa, 45 kDa
• Numéro d’acquisition GenBank: BC016925
• Symbole du gène: NRF1
• Identification du gène (NCBI): 4899
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NRF1, also named as Alpha-pal and NRF-1, belongs to the NRF1/Ewg family. It is a transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. NRF1 is implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. It plays a critical role in regulating the expression of many antioxidant response element (ARE)-dependent genes. NRF1 exists various isoforms and long isoform is about 67 kDa and short isoform is about 45 kDa.

Publications

Species	Application	Title
mouse	WB	Nature; A plant-derived natural photosynthetic system for improving cell anabolism; Authors - Pengfei Chen
chicken	WB,IHC	J Hazard Mater; Mitochondrial miR-12294-5p regulated copper-induced mitochondrial oxidative stress and mitochondrial quality control imbalance by targeted inhibition of CISD1 in chicken livers; Authors - Gaolong Zhong
	WB	J Autoimmun; Autoantibodies against the NineTeen complex and U5 RNP in systemic sclerosis; Authors - Jean-Baptiste Vulsteke
rat,human	WB	J Adv Res; A dual absorption pathway of novel oyster-derived peptide-zinc complex enhances zinc bioavailability and restores mitochondrial function; Authors - Ximing Yang
human	WB	Redox Biol; Defective mitochondrial ISCs biogenesis switches on IRP1 to fine tune selective mitophagy.; Authors - Hao Wu
chicken	WB	Cell Commun Signal; Local GHR roles in regulation of mitochondrial function through mitochondrial biogenesis during myoblast differentiation; Authors - Bowen Hu

{{ptg:RelatedPrimaryAntibodies}}