Anticorps Polyclonal de lapin anti-NTHL1

NTHL1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications(4)

Avis (1)

N° de cat : 11154-1-AP

Synonymes

NTH1, NTHL1, OCTS3

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Galerie de données de validation

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  WB analysis using 11154-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11154-1-AP (NTHL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11154-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11154-1-AP (NTHL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11154-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11154-1-AP (NTHL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules K-562, tissu cérébral de rat, tissu cérébral humain

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IP: See 1 publications below

Informations sur le produit

11154-1-AP cible NTHL1 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NTHL1 Protéine recombinante Ag1631
• Nom complet: nth endonuclease III-like 1 (E. coli)
• Masse moléculaire calculée: 34 aa, 3 kDa
• Poids moléculaire observé: 34 kDa
• Numéro d’acquisition GenBank: BC003014
• Symbole du gène: NTHL1
• Identification du gène (NCBI): 4913
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NTHL1, also named as Endonuclease III-like protein 1 or OCTS3, is a 312 amino acid protein, which belongs to the Nth/MutY family. NTHL1 localizes in the nucleus and is widely expressed with highest levels in heart and lowest levels in lung and liver. NTHL1 has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity and incises damaged DNA at cytosines, thymines and guanines. NTHL1 acts on a damaged strand, 5' from the damaged site and is required for the repair of both oxidative DNA damage and spontaneous mutagenic lesions.

Publications

Species	Application	Title
rat	WB	FASEB J; Chronic gestational hypoxia accelerates ovarian aging and lowers ovarian reserve in next-generation adult rats.; Authors - Catherine E Aiken
human	WB	Respir Res; Protection of pulmonary epithelial cells from oxidative stress by hMYH adenine glycosylase.; Authors - Kremer Ted M TM
human	WB,IP	NAR Cancer; The DNA repair function of BCL11A suppresses senescence and promotes continued proliferation of triple-negative breast cancer cells; Authors - Elise Vickridge
human	WB	Nucleic Acids Res; The function of BCL11B in base excision repair contributes to its dual role as an oncogene and a haplo-insufficient tumor suppressor gene; Authors - Elise Vickridge

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

James (Verified Customer) (02-17-2023)

Recognises NTHL1 at 34kDa, but has non-specific bands which decrease with siRNA knock-down of NTHL1 at higher molecular weights (150kDa).

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: U2OS

NTHL1 Antibody Western Blot validation (1:1000 dilution) in U2OS (Cat no:11154-1-AP)

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