Anticorps Polyclonal de lapin anti-NUB1

NUB1 Polyclonal Antibody for IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 14343-1-AP

Synonymes

BS4, NEDD8 ultimate buster 1, NUB1, NUB1L, NYREN18

---

Galerie de données de validation

• 

  WB analysis of HeLa using 14343-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14343-1-AP (NUB1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14343-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14343-1-AP (NUB1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 14343-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14343-1-AP (NUB1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 14343-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14343-1-AP (NUB1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 14343-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14343-1-AP (NUB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 14343-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 14343-1-AP (NUB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 14343-1-AP

  IP Result of anti-NUB1 (IP:14343-1-AP, 3ug; Detection:14343-1-AP 1:600) with HEK-293 cells lysate 2500ug.

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules COLO 320, cellules HepG2, cellules K-562, cellules Raji, tissu rénal de souris
• Résultats positifs en IP: cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

14343-1-AP cible NUB1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NUB1 Protéine recombinante Ag5668
• Nom complet: negative regulator of ubiquitin-like proteins 1
• Masse moléculaire calculée: 71 kDa
• Poids moléculaire observé: 69 kDa
• Numéro d’acquisition GenBank: BC046354
• Symbole du gène: NUB1
• Identification du gène (NCBI): 51667
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Proteasome-associated protein NEDD8 ultimate buster 1 (NUB1) is an interferon-inducible protein that down-regulates NEDD8 expression and its conjugation system. Over-expression of NUB1 induces a growth-inhibitory effect in cells, and it mediates anti-proliferative actions and apoptosis in renal cell carcinoma cells through cell-cycle regulation. Expression of NUB1 leads to decreased modification of p53 with NEDD8 and stimulation of p53 ubiquitination. Recent finding revealed that NUB1, by regulating GSK3β levels, modulates tau phosphorylation and aggregation, and is a key player in neurodegeneration associated with tau pathology.

Publications

Species	Application	Title
mouse	WB,IF	Biochem Biophys Res Commun; The role of NUB1 in α-synuclein degradation in Lewy body disease model mice.; Authors - Kunikazu Tanji
human	IHC	Neuropathology; Ubiquitin-negative, eosinophilic neuronal cytoplasmic inclusions associated with stress granules and autophagy: an immunohistochemical investigation of two cases.; Authors - Fumiaki Mori
human	WB	Brain Pathol; Phosphorylated NUB1 distinguishes α-synuclein in Lewy bodies from that in glial cytoplasmic inclusions in multiple system atrophy; Authors - Kunikazu Tanji

Produits associés

AIPL1 Polyclonal antibody

15108-1-AP

P53 Monoclonal antibody

60283-1-Ig

P53 Polyclonal antibody

21891-1-AP

NEDD8 Polyclonal antibody

16777-1-AP

PSMD4 Monoclonal antibody

66179-1-Ig

SNCAIP Polyclonal antibody

17818-1-AP

UBE2K Polyclonal antibody

11834-3-AP