Anticorps Recombinant de lapin anti-NUCB2/nesfatin-1

NUCB2/nesfatin-1 Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

241037C1

N° de cat : 83917-5-RR

Synonymes

NUCB2, 241037C1, DNA binding protein NEFA, Gastric cancer antigen Zg4, NEFA

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Galerie de données de validation

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  IF Staining of HepG2 using 83917-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NUCB2 antibody (83917-5-RR, Clone: 241037C1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HepG2 using 83917-5-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NUCB2/nesfatin-1 antibody (83917-5-RR, Clone: 241037C1 ) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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  FC experiment of A431 using 83917-5-RR

  1x10^6 A431 cells were intracellularly stained with 0.25 ug NUCB2 Recombinant antibody (83917-5-RR, Clone:241037C1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of HepG2 using 83917-5-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug NUCB2 Recombinant antibody (83917-5-RR, Clone:241037C1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules A431, cellules HepG2

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83917-5-RR cible NUCB2/nesfatin-1 dans les applications de IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NUCB2/nesfatin-1 Protéine recombinante Ag25152
• Nom complet: nucleobindin 2
• Masse moléculaire calculée: 50 kDa
• Numéro d’acquisition GenBank: NM_005013
• Symbole du gène: NUCB2
• Identification du gène (NCBI): 4925
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NucB2, an anorexigenic molecule, is expressed mainly in the hypothalamus, particularly in the supraoptic nucleus (SON) and the paraventricular nucleus (PVN). NucB2 is also expressed in the subfornical organ (SFO). Because the SON and PVN are involved in body fluid regulation, NucB2 may be involved in dehydration-induced anorexia. NUCB2 is composed of a signal peptide of 24 amino acids in the N-terminal region and a protin structure containing 396 amino acids. Nesfatin-1 is an 82-amino-acid peptide hormone cleaved from nucleobindin2 (NUCB2).

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