Anticorps Polyclonal de lapin anti-NUDCD2

• Phare
• Validé par KD/KO

NUDCD2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 21205-1-AP

Synonymes

NudCL2, NudC domain-containing protein 2, NudC domain containing 2

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Galerie de données de validation

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  WB analysis using 21205-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21205-1-AP (NUDCD2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 21205-1-AP

  WB result of NUDCD2 antibody (21205-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NUDCD2 transfected HEK-293 cells.

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  WB analysis of human brain using 21205-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21205-1-AP (NUDCD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 21205-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21205-1-AP (NUDCD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 21205-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21205-1-AP (NUDCD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 21205-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 21205-1-AP (NUDCD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 21205-1-AP

  IP result of anti-NUDCD2 (IP:21205-1-AP, 4ug; Detection:21205-1-AP 1:500) with HepG2 cells lysate 1080 ug.

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  IHC staining of human kidney using 21205-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21205-1-AP (NUDCD2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 21205-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21205-1-AP (NUDCD2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 21205-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21205-1-AP (NUDCD2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 21205-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 21205-1-AP (NUDCD2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human breast cancer using 21205-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 21205-1-AP (NUDCD2 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 21205-1-AP

  Immunofluorescent analysis of HepG2 cells, using NUDCD2 antibody 21205-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HepG2 using 21205-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NUDCD2 antibody (21205-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 21205-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NUDCD2 antibody (21205-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of HepG2 using 21205-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NUDCD2 antibody (21205-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  IF Staining of HeLa using 21205-1-AP

  Immunofluorescent analysis of Hela cells, using NUDCD2 antibody 21205-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HEK-293, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain, tissu rénal humain
• Résultats positifs en IP: cellules HepG2,
• Résultats positifs en IHC: tissu rénal humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

21205-1-AP cible NUDCD2 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NUDCD2 Protéine recombinante Ag15579
• Nom complet: NudC domain containing 2
• Masse moléculaire calculée: 157 aa, 18 kDa
• Poids moléculaire observé: 18 kDa
• Numéro d’acquisition GenBank: BC017934
• Symbole du gène: NUDCD2
• Identification du gène (NCBI): 134492
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NUDCD2, also known as NudCL2, is an HSP90 cochaperone that regulates sister chromatid cohesion during mitosis. NUDCD2 also plays a role in ciliogenesis(PMID: 30368549, 34480124). NUDCD2 regulates the LIS1/dynein pathway by stabilizing LIS1 with Hsp90 chaperone(PMID: 20133715).

Publications

Species	Application	Title
mouse	WB	Cell Mol Life Sci; RetSat stabilizes mitotic chromosome segregation in pluripotent stem cells; Authors - Wanzhi Cai

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