Anticorps Polyclonal de lapin anti-NUDT21

• Phare
• Validé par KD/KO

NUDT21 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, IP, CoIP, ChIP, RIP, ELISA

Conjugaison

Non conjugué

Publications(39)

Avis (2)

N° de cat : 10322-1-AP

Synonymes

CFIm25, Cleavage factor Im complex 25 kDa subunit, CPSF25, CPSF5, Nucleoside diphosphate-linked moiety X motif 21

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 10322-1-AP

  WB result of NUDT21 antibody (10322-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NUDT21 transfected NIH/3T3 cells.

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  WB analysis using 10322-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10322-1-AP (NUDT21 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 10322-1-AP

  IP result of anti-NUDT21 (IP:10322-1-AP, 4ug; Detection:10322-1-AP 1:2000) with MCF-7 cells lysate 640 ug.

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  IHC staining of human colon cancer using 10322-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10322-1-AP (NUDT21 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10322-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10322-1-AP (NUDT21 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 10322-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using NUDT21 antibody (10322-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 10322-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NUDT21 antibody (10322-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules MCF-7, cellules NIH/3T3
• Résultats positifs en IP: cellules MCF-7,
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris,
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 10 publications below
• WB: See 34 publications below
• IHC: See 6 publications below
• IF: See 4 publications below
• IP: See 4 publications below
• CoIP: See 1 publications below
• ChIP: See 2 publications below
• RIP: See 3 publications below

Informations sur le produit

10322-1-AP cible NUDT21 dans les applications de WB, IHC, IF/ICC, IF-P, IP, CoIP, ChIP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NUDT21 Protéine recombinante Ag0393
• Nom complet: nudix (nucleoside diphosphate linked moiety X)-type motif 21
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 26 kDa
• Numéro d’acquisition GenBank: BC001403
• Symbole du gène: NUDT21
• Identification du gène (NCBI): 11051
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cleavage and polyadenylation specific factor 5, 25 kDa (CPSF5, synonym: CFIM25) is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of CPSF5 with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors.

Publications

Species	Application	Title
human	WB	Nature; CFIm25 links alternative polyadenylation to glioblastoma tumour suppression.; Authors - Chioniso P Masamha ; KD Validated
human	WB	Mol Cell; A Complex of U1 snRNP with Cleavage and Polyadenylation Factors Controls Telescripting, Regulating mRNA Transcription in Human Cells.; Authors - Byung Ran So ; KD Validated
	WB	Mol Cell; Global Promotion of Alternative Internal Exon Usage by mRNA 3' End Formation Factors.; Authors - Ashish Misra
human	WB	Sci Adv; Alternative polyadenylation is a determinant of oncogenic Ras function.; Authors - Aishwarya Subramanian
human	IHC	J Exp Med; Downregulation of CFIm25 amplifies dermal fibrosis through alternative polyadenylation.; Authors - Tingting Weng
mouse	WB	EMBO J; The Mediator subunit MED23 couples H2B mono-ubiquitination to transcriptional control and cell fate determination.; Authors - Xiao Yao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Shahrazad (Verified Customer) (07-23-2025)

Used for IF of mouse brain samples along with NeuN and DAPI. Troubleshooting for high background problem.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mouse brain

FH

Fabienne (Verified Customer) (05-09-2025)

it works very well in western blot (tested on nuclear protein extracts)

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Jurkat

NUDT21 Antibody Western Blot validation (1:1000 dilution) in Jurkat (Cat no:10322-1-AP)

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