• Phare
  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-NUF2

NUF2 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(8)

N° de cat : 15731-1-AP

Synonymes

CDCA1, hNuf2, hNuf2R, hsNuf2, Kinetochore protein Nuf2, NUF2, NUF2R



Applications testées

Résultats positifs en WBcellules HEK-293, cellules HeLa, cellules HepG2, cellules PC-3, tissu de muscle squelettique humain, tissu hépatique humain, tissu testiculaire humain
Résultats positifs en IPcellules HEK-293

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

15731-1-AP cible NUF2 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Réactivité citéeHumain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène NUF2 Protéine recombinante Ag8479
Nom complet NUF2, NDC80 kinetochore complex component, homolog (S. cerevisiae)
Masse moléculaire calculée 464 aa, 54 kDa
Poids moléculaire observé 50 kDa
Numéro d’acquisition GenBankBC008489
Symbole du gène NUF2
Identification du gène (NCBI) 83540
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS with 0.02% sodium azide and 50% glycerol
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NUF2 (NUF2, Ndc80 kinetochore complex component) plays an important role in kinetochore-microtubule attachment. NUF2 was reported to be overexpressed in various kinds of carcinomas including lung cancer, cholangiocellular cancer, urinary bladder cancer, renal cell cancer, colorectal cancer, gastric cancer, ovarian cancer and serous adenocarcinoma. Elevated expression of NUF2 was associated with poorer prognosis of non-small cell lung cancer patients (PMID: 17079454). Knockdown of NUF2 significantly caused cell growth delay and increased apoptosis in cell lines of non-small cell lung cancer, colorectal cancer, gastric cancer and ovarian cancer.

Protocole

Product Specific Protocols
WB protocol for NUF2 antibody 15731-1-APDownload protocol
IP protocol for NUF2 antibody 15731-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Dis

LncRNA CDKN2B-AS1 stabilized by IGF2BP3 drives the malignancy of renal clear cell carcinoma through epigenetically activating NUF2 transcription.

Authors - Xina Xie
humanWB,IHC

Int J Biol Sci

NUF2 Drives Clear Cell Renal Cell Carcinoma by Activating HMGA2 Transcription through KDM2A-mediated H3K36me2 Demethylation

Authors - Jiatian Lin
  • KD Validated
human,mouseWB

Front Oncol

NUF2 overexpression contributes to epithelial ovarian cancer progression via ERBB3-mediated PI3K-AKT and MAPK signaling axes

Authors - Ruobing Leng
  • KD Validated
humanWB

Adv Sci (Weinh)

CCDC68 Maintains Mitotic Checkpoint Activation by Promoting CDC20 Integration into the MCC

Authors - Qi Li
humanWB,IHC

Carcinogenesis

NUF2 and NEK2 promote malignant progression of gallbladder cancer by remodeling the extracellular matrix

Authors - Ming Gao
humanWB,IP,IHC

Biochem Pharmacol

NUF2 activated by YY1 promotes prostate cancer malignancy via p38/MAPK signaling axis and serves as a therapeutic target

Authors - Chengqi Jin

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Katie (Verified Customer) (05-23-2022)

Works well for western

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: T cells
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