Anticorps Polyclonal de lapin anti-NUMBL

• Phare
• Validé par KD/KO

NUMBL Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 10111-1-AP

Synonymes

CAG3A, CTG3a, NBL, numb homolog (Drosophila) like, Numb like protein, NUMB R, Numb related protein, NUMBL, NUMBLIKE, NUMBR, TNRC23

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Galerie de données de validation

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  WB analysis of mouse brain using 10111-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10111-1-AP (NUMBL antibody at dilution of 1:2000 incubated at 4 degree celsius over night.

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  WB analysis of HeLa using 10111-1-AP

  WB result of NUMBL antibody (10111-1-AP, 1:3000) with si-Control and si-NUMBL transfected HeLa cells.

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  WB analysis of mouse lung using 10111-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 10111-1-AP (NUMBL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 10111-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10111-1-AP (NUMBL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 10111-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 10111-1-AP (NUMBL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10111-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10111-1-AP (NUMBL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10111-1-AP

  IP result of anti-NUMBL (IP:10111-1-AP, 4ug; Detection:10111-1-AP 1:1000) with HeLa cells lysate 2800 ug.

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  IHC staining of human brain using 10111-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10111-1-AP (NUMBL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 10111-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10111-1-AP (NUMBL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 10111-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10111-1-AP (NUMBL antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 10111-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10111-1-AP (NUMBL antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of PC-12 using 10111-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using 10111-1-AP (NUMBL antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 10111-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 10111-1-AP (NUMBL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HeLa, tissu hépatique de souris, tissu pulmonaire de souris, tissu splénique de souris
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu cérébral humain, tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules PC-12, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 3 publications below

Informations sur le produit

10111-1-AP cible NUMBL dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NUMBL Protéine recombinante Ag0156
• Nom complet: numb homolog (Drosophila)-like
• Masse moléculaire calculée: 65 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC001794
• Symbole du gène: NUMBL
• Identification du gène (NCBI): 9253
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Numbl (also known as numblike or Nbl), as a conserved homolog of Drosophila Numb, is specifically expressed in the brain. Numbl is a cytoplasm protein and has redundant functions in embryonic neurogenesis. It plays an important role in ependymal wall integrity and subventricular zone neuroblast survival. However, its expression and function in the central nervous system lesion are still unclear.

Publications

Species	Application	Title
mouse	WB	Nat Neurosci; Numb and Numbl are required for maintenance of cadherin-based adhesion and polarity of neural progenitors.; Authors - Rasin Mladen-Roko MR
human	WB,IHC	Cancer Res; Regulation of lung cancer metastasis by Klf4-Numb-like signaling.; Authors - Vaira Valentina V ; KD Validated
human	WB,IHC	J Clin Endocrinol Metab; PMID: 16705073; Authors - Sinha-Hikim Indrani I
mouse	WB	Development; Cleaved Delta like 1 intracellular domain regulates neural development via Notch signal-dependent and -independent pathways.; Authors - Yusuke Okubo
mouse	IHC	Mol Endocrinol; Numb deletion in POMC-expressing cells impairs pituitary intermediate lobe cell adhesion, progenitor cell localization, and neuro-intermediate lobe boundary formation.; Authors - Moran Tyler B TB