Anticorps Polyclonal de lapin anti-NUP50

• Phare
• Validé par KD/KO

NUP50 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 20798-1-AP

Synonymes

50 kDa nucleoporin, nucleoporin 50kDa, Nucleoporin Nup50, NUP50

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Galerie de données de validation

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  WB analysis of Jurkat using 20798-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20798-1-AP (NUP50 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 20798-1-AP

  WB result of NUP50 antibody (20798-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NUP50 transfected HEK-293 cells.

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  WB analysis of mouse brain using 20798-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20798-1-AP (NUP50 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 20798-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20798-1-AP (NUP50 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 20798-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20798-1-AP (NUP50 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 20798-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20798-1-AP (NUP50 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 20798-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20798-1-AP (NUP50 antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HEK-293, tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:2500-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 3 publications below

Informations sur le produit

20798-1-AP cible NUP50 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NUP50 Protéine recombinante Ag14710
• Nom complet: nucleoporin 50kDa
• Masse moléculaire calculée: 468 aa, 50 kDa
• Poids moléculaire observé: 50 kDa, 65 kDa
• Numéro d’acquisition GenBank: BC028125
• Symbole du gène: NUP50
• Identification du gène (NCBI): 10762
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Nup50 (also known as Npap60) is a nucleoporin that binds directly to importin α and their interaction plays an important role in nuclear import. Nup50 is a mobile nucleoporin localized primarily in the nuclear basket of the nuclear pore complex (NPC) and in the nucleoplasm. Human Nup50 has two isoforms: Npap60L (1-469 aa), and its alternatively spliced isoform, Npap60S (29-469 aa) of Npap60L. This antibody recognizes both of two isoforms.

Publications

Species	Application	Title
human	WB	Oncogene; DNA damage-induced translocation of mitochondrial factor HIGD1A into the nucleus regulates homologous recombination and radio/chemo-sensitivity.; Authors - Bin Chen
rat	WB	J Cell Sci; Nucleoporin 50 mediates Kcna4 transcription to regulate cardiac electrical activity.; Authors - Xueting Gao ; KD Validated
human	WB	Nat Phys; Structural anisotropy results in mechano-directional transport of proteins across nuclear pores; Authors - Fani Panagaki ; KD Validated

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