Anticorps Polyclonal de lapin anti-NUSAP1

• Phare
• Validé par KD/KO

NUSAP1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

37

N° de cat : 12024-1-AP

Synonymes

ANKT, BM037, LNP, NuSAP, NUSAP1, PRO0310, PRO0310p1, Q0310, SAPL

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Galerie de données de validation

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  WB analysis using 12024-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12024-1-AP (NUSAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 12024-1-AP

  IP Result of anti-NUSAP1 (IP:12024-1-AP, 4ug; Detection:12024-1-AP 1:500) with HeLa cells lysate 2000ug.

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  IHC staining of human prostate cancer using 12024-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12024-1-AP (NUSAP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 12024-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12024-1-AP (NUSAP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12024-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12024-1-AP (NUSAP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12024-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12024-1-AP (NUSAP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 12024-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NUSAP1 antibody (12024-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules Jurkat
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 26 publications below
• IHC: See 14 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Informations sur le produit

12024-1-AP cible NUSAP1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NUSAP1 Protéine recombinante Ag2654
• Nom complet: nucleolar and spindle associated protein 1
• Masse moléculaire calculée: 440 aa, 49 kDa
• Poids moléculaire observé: 47-52 kDa
• Numéro d’acquisition GenBank: BC001308
• Symbole du gène: NUSAP1
• Identification du gène (NCBI): 51203
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NUSAP1, also named as ANKT, belongs to the NUSAP family. It is microtubule-associated protein with the capacity to bundle and stabilize microtubules. NUSAP1 may associate with chromosomes and promote the organization of mitotic spindle microtubules around them. NUSAP1 plays a role in connecting apoptosis with cell migration and it is a potentially involved in morphogenesis in vertebrates (PMID:21203972). NUSAP1 is an immunogenic antigen in 65% of patients with AML following allogeneic HCT and suggests a tumor antigen role(PMID:20053754 ). NuSAP1 protein level is tightly regulated by the APC/C ubiquitin ligase complex and NuSAP induces mitotic arrest dependent of its microtubule affinity (PMID:17618083). NUSAP1 is overexpressed in pituitary adenomas.

Publications

Species	Application	Title
human	WB	Cell; Enhanced protein degradation by branched ubiquitin chains.; Authors - Hermann-Josef Meyer
human	IF	Gastroenterology; microRNA 193a-5p Regulates Levels of Nucleolar- and Spindle-Associated Protein 1 to Suppress Hepatocarcinogenesis.; Authors - Sanchari Roy
human	WB	Mol Cell; Regulated degradation of spindle assembly factors by the anaphase-promoting complex.; Authors - Ling Song
human	WB	Mol Cell; Microtubule-dependent regulation of mitotic protein degradation.; Authors - Ling Song
human	IHC, IF	J Exp Med; Single-cell transcriptome analysis reveals differential nutrient absorption functions in human intestine.; Authors - Yalong Wang
human, rat	IHC	Acta Neuropathol; Transcriptome analysis of MENX-associated rat pituitary adenomas identifies novel molecular mechanisms involved in the pathogenesis of human pituitary gonadotroph adenomas.; Authors - Lee Misu M

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