Anticorps Polyclonal de lapin anti-NXF1

• Phare
• Validé par KD/KO

NXF1 Polyclonal Antibody for IF, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IP, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 10328-1-AP

Synonymes

MEX67, mRNA export factor TAP, nuclear RNA export factor 1, NXF1, TAP, Tip associated protein, Tip associating protein

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Galerie de données de validation

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  WB analysis of HeLa using 10328-1-AP

  WB result of NXF1 antibody (10328-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NXF1 transfected HeLa cells.

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  WB analysis of HeLa using 10328-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10328-1-AP (NXF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 10328-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10328-1-AP (NXF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10328-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10328-1-AP (NXF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 10328-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10328-1-AP (NXF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10328-1-AP

  IP result of anti-NXF1 (IP:10328-1-AP, 4ug; Detection:10328-1-AP 1:500) with HeLa cells lysate 3000 ug.

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  IF Staining of HepG2 using 10328-1-AP

  Immunofluorescent analysis of HepG2 cells using 10328-1-AP (NXF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293T, cellules HepG2, cellules K-562
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 8 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

10328-1-AP cible NXF1 dans les applications de WB, IP, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NXF1 Protéine recombinante Ag0413
• Nom complet: nuclear RNA export factor 1
• Masse moléculaire calculée: 70 kDa
• Poids moléculaire observé: 66-70 kDa
• Numéro d’acquisition GenBank: BC004904
• Symbole du gène: NXF1
• Identification du gène (NCBI): 10482
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Nuclear RNA export factor 1 (NXF1, synonyms: TAP, MEX67) is one member of a family of nuclear RNA export factors. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1(NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. This protein shuttles between the nucleus and the cytoplasm and binds in vivo to poly(A)+ RNA. It is the vertebrate homologue of the yeast protein Mex67p. NXF1 overcomes the mRNA export block caused by the presence of saturating amounts of CTE (constitutive transport element) RNA of type D retroviruses. There are some phosphorylation modification sites center on the N-terminal of NXF1.

Publications

Species	Application	Title
human	WB	J Cell Biol; Inhibition of pyrimidine synthesis reverses viral virulence factor-mediated block of mRNA nuclear export.; Authors - Zhang Liang L ; KD Validated
		J Cell Biol; Intronless mRNAs transit through nuclear speckles to gain export competence.; Authors - Ke Wang
human	WB	Nucleic Acids Res; Depletion of REF/Aly alters gene expression and reduces RNA polymerase II occupancy.; Authors - Sarah H Stubbs
human	WB	PLoS Genet; Nucleoporin TPR promotes tRNA nuclear export and protein synthesis in lung cancer cells.; Authors - Miao Chen ; KD Validated
monkey	WB,CoIP	Mol Cell Biol; Fragile X mental retardation protein FMRP binds mRNAs in the nucleus.; Authors - Kim Miri M ; KD Validated
human	WB,IF,IP	Chromosoma; XIST RNA exhibits nuclear retention and exhibits reduced association with the export factor TAP/NXF1.; Authors - Cohen Hannah R HR