Anticorps Polyclonal de lapin anti-TXNL6

TXNL6 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 11203-1-AP

Synonymes

nucleoredoxin like 1, Nucleoredoxin like protein 1, NXNL1, RDCVF, Thioredoxin like protein 6, TXNL6

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Galerie de données de validation

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  WB analysis of mouse brain using 11203-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11203-1-AP (TXNL6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11203-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11203-1-AP (TXNL6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 11203-1-AP

  IP result of anti-TXNL6 (IP:11203-1-AP, 4ug; Detection:11203-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of human breast cancer using 11203-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11203-1-AP (TXNL6 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human breast cancer using 11203-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 11203-1-AP (TXNL6 Antibody) at dilution of 1:50.

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  IHC staining of human breast cancer using 11203-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 11203-1-AP (TXNL6 Antibody) at dilution of 1:50.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HeLa
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

11203-1-AP cible TXNL6 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, porc
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TXNL6 Protéine recombinante Ag1691
• Nom complet: nucleoredoxin-like 1
• Masse moléculaire calculée: 24 kDa
• Poids moléculaire observé: 26-28 kDa
• Numéro d’acquisition GenBank: BC014127
• Symbole du gène: NXNL1
• Identification du gène (NCBI): 115861
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TXNL6, also named as NXNL1, belongs to the nucleoredoxin family. It may play a role in cone cell viability, slowing down cone degeneration, does not seem to play a role in degenerating rods. TXNL6 is a Novel Oxidative Stress-Induced Reducing System for Methionine Sulfoxide Reductase A Repair of a-Crystallin and Cytochrome C in the Eye Lens. It serve as a reducing system for MsrA repair of the essential lens chaperone a-crystallin/sHSP and mitochondrial cytochrome c. TXNL6 is induced at high levels in human lens epithelial cells exposed to H2O2-induced oxidative stress.(PMID:21079812)

Publications

Species	Application	Title
pig	IF	Cell Rep; Two-Step Reactivation of Dormant Cones in Retinitis Pigmentosa.; Authors - Wei Wang
human	WB,IF	PLoS One; TXNL6 is a novel oxidative stress-induced reducing system for methionine sulfoxide reductase a repair of α-crystallin and cytochrome C in the eye lens.; Authors - Brennan Lisa A LA

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