Anticorps Recombinant de lapin anti-Neutrophil Elastase

Neutrophil Elastase Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC (Intra)

Conjugaison

Non conjugué

CloneNo.

250346G5

N° de cat : 98499-1-RR

Synonymes

ELA2, ELANE, EC:3.4.21.37, Elastase 2, HLE

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Galerie de données de validation

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  FC experiment of human peripheral blood leukocytes using 98499-1-RR

  1x10^6 human peripheral blood leukocytes were intracellularly stained with 0.25 ug Anti-Human Neutrophil Elastase Rabbit RecAb (98499-1-RR, Clone: 250346G5) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of U-937 using 98499-1-RR

  1x10^6 U-937 cells (left) or Jurkat cells (right) were intracellularly stained with 0.25 ug Anti-Human Neutrophil Elastase Rabbit RecAb (98499-1-RR, Clone: 250346G5) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en FC (Intra): human peripheral blood leukocytes, cellules U-937

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98499-1-RR cible Neutrophil Elastase dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Neutrophil Elastase Protéine recombinante Eg3192
• Nom complet: elastase 2, neutrophil
• Masse moléculaire calculée: 29KD
• Numéro d’acquisition GenBank: NM_001972.4
• Symbole du gène: ELA2
• Identification du gène (NCBI): 1991
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

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