Anticorps Recombinant de lapin anti-Neutrophil elastase

Neutrophil elastase Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC (Intra)

Conjugaison

FITC Plus Fluorescent Dye

CloneNo.

241842G3

N° de cat : FITC-98197

Synonymes

241842G3, EC:3.4.21.37, Ela2, Elane, Elastase-2

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Galerie de données de validation

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  FC experiment of mouse bone marrow cells using FITC-98197

  1x10^6 mouse bone marrow cells (left) or NIH/3T3 cells (right) were intracellularly stained with 0.25 ug FITC Plus Anti-Mouse Neutrophil elastase Rabbit RecAb (FITC-98197, Clone: 241842G3) (red) or FITC Plus Rabbit IgG Isotype Control RecAb (FITC-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en FC (Intra): cellules médullaires de souris,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

FITC-98197 cible Neutrophil elastase dans les applications de FC (Intra) et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Neutrophil elastase Protéine recombinante Eg2126
• Nom complet: elastase 2, neutrophil
• Masse moléculaire calculée: 29KD
• Numéro d’acquisition GenBank: NM_015779.2
• Symbole du gène: Ela2
• Identification du gène (NCBI): 50701
• Conjugaison: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

ELA2 (Neutrophil elastase) is also named as ELAL, NE, HLE, HNE, PMN-E, SERP1, GE, ELANE and belongs to the peptidase S1 family. It is a 33-kDa enzyme with several isoforms that differ in their extent of glycosylation (PMID:12223222). Human neutrophil elastase (HNE) is a serine protease with potent proteolytic activity (3), which is reported to cause mucin secretion (degranulation) (PMID:12169572). It may contribute to the directed migration of leukocytes into flamed postcapillary venules in addition to contributing to the process of tissue emigration (PMID:9683424). The full length protein has a signal peptide,propeptide and three glycosylation sites.

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