Anticorps Monoclonal anti-Ninein

• Phare
• Validé par KD/KO

Ninein Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

canin, Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(3)

CloneNo.

1B10A8

Avis (2)

N° de cat : 67132-1-Ig

Synonymes

NIN, 1B10A8, Glycogen synthase kinase 3 beta-interacting protein, GSK3B interacting protein, GSK3B-interacting protein

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Galerie de données de validation

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  WB analysis using 67132-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67132-1-Ig (Ninein antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 67132-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67132-1-Ig (Ninein antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67132-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67132-1-Ig (Ninein antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 67132-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67132-1-Ig (Ninein antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 67132-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67132-1-Ig (Ninein antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MDCK using 67132-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using 67132-1-Ig (Ninein antibody) at dilution of 1:500 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HT-29, cellules MCF-7, cellules NCCIT
• Résultats positifs en IHC: tissu cérébral de souris, tissu cardiaque de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MDCK,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 3 publications below

Informations sur le produit

67132-1-Ig cible Ninein dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons canin, Humain, souris

• Réactivité: canin, Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Ninein Protéine recombinante Ag14652
• Nom complet: ninein (GSK3B interacting protein)
• Masse moléculaire calculée: 2090 aa, 243 kDa
• Poids moléculaire observé: 220 kDa
• Numéro d’acquisition GenBank: BC034708
• Symbole du gène: Ninein
• Identification du gène (NCBI): 51199
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ninein is a large coiled-coil protein that associates with the subdistal appendages of the mother centriole and the minus-ends of both centrioles. Ninein interacts with γ-tubulin and microtubule nucleation and anchoring at the centrosomes.

Publications

Species	Application	Title
human	WB,IF	Dev Cell; Proteomic profiling of centrosomes across multiple mammalian cell and tissue types by an affinity capture method; Authors - Sarah Carden ; KO Validated
human	IF	J Cell Sci; Roles for CEP170 in cilia function and dynein-2 assembly; Authors - Johannes F Weijman
	IF	Elife; Trisomy 21 induces pericentrosomal crowding delaying primary ciliogenesis and mouse cerebellar development; Authors - Cayla E Jewett

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Elisa (Verified Customer) (07-12-2021)

This antibody stains well ninein pool at the centrosome, in MetOH fixation conditions

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:300 IF 1:500WB
• Cell Tissue Type: HAP1, H9, RPE1 and HEK293T cells

FH

Sarah (Verified Customer) (07-06-2020)

Antibody used at 1:1000 to visualise ninein protein (from 20ug cell lysate) in Hap1 control and Ninein KO cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Hap1

Ninein Antibody Western Blot validation (1:1000 dilution) in Hap1 (Cat no:67132-1-Ig)

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