Anticorps Polyclonal de lapin anti-NUCB2/nesfatin-1

NUCB2/nesfatin-1 Polyclonal Antibody for IF, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

2

Avis (1)

N° de cat : 26712-1-AP

Synonymes

DNA binding protein NEFA, Gastric cancer antigen Zg4, NEFA, NUCB2, nucleobindin 2

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Galerie de données de validation

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  WB analysis using 26712-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26712-1-AP (NUCB2/nesfatin-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 26712-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 26712-1-AP (Nucleobindin 2 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 26712-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 26712-1-AP (Nucleobindin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 26712-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 26712-1-AP (Nucleobindin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 26712-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 26712-1-AP (NUCB2/nesfatin-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 26712-1-AP

  Human milk were subjected to SDS PAGE followed by western blot with 26712-1-AP (NUCB2/nesfatin-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 26712-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 26712-1-AP (Nucleobindin 2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HEK-293T, cellules HepG2, lait humain, tissu cérébral de rat, tissu testiculaire de souris
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

26712-1-AP cible NUCB2/nesfatin-1 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NUCB2/nesfatin-1 Protéine recombinante Ag25152
• Nom complet: nucleobindin 2
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: NM_005013
• Symbole du gène: NUCB2
• Identification du gène (NCBI): 4925
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NucB2, an anorexigenic molecule, is expressed mainly in the hypothalamus, particularly in the supraoptic nucleus (SON) and the paraventricular nucleus (PVN). NucB2 is also expressed in the subfornical organ (SFO). Because the SON and PVN are involved in body fluid regulation, NucB2 may be involved in dehydration-induced anorexia. NUCB2 is composed of a signal peptide of 24 amino acids in the N-terminal region and a protin structure containing 396 amino acids. Nesfatin-1 is an 82-amino-acid peptide hormone cleaved from nucleobindin2 (NUCB2).

Publications

Species	Application	Title
human	WB	Proteomics; PMID: 29901268; Authors - Wang Li
human	WB	J Transl Med; NUCB2/Nesfatin-1 drives breast cancer metastasis through the up-regulation of cholesterol synthesis via the mTORC1 pathway; Authors - Siyi Ning

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Guorong (Verified Customer) (11-22-2022)

Clear and specific band

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: ARPE-19

NUCB2/nesfatin-1 Antibody Western Blot validation (1:500 dilution) in ARPE-19 (Cat no:26712-1-AP)