Anticorps Polyclonal de lapin anti-NUCB2/nesfatin-1

NUCB2/nesfatin-1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(3)

Avis (1)

N° de cat : 26712-1-AP

Synonymes

NUCB2, DNA binding protein NEFA, Gastric cancer antigen Zg4, NEFA, nucleobindin 2

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Galerie de données de validation

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  WB analysis using 26712-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26712-1-AP (NUCB2/nesfatin-1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 26712-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 26712-1-AP (Nucleobindin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 26712-1-AP

  Human milk were subjected to SDS PAGE followed by western blot with 26712-1-AP (NUCB2/nesfatin-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 26712-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 26712-1-AP (NUCB2/nesfatin-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 26712-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26712-1-AP (NUCB2/nesfatin-1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 26712-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 26712-1-AP (NUCB2/nesfatin-1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 26712-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 26712-1-AP (NUCB2/nesfatin-1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 26712-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26712-1-AP (NUCB2/nesfatin-1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 26712-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NUCB2/nesfatin-1 antibody (26712-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of HepG2 using 26712-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug NUCB2/nesfatin-1 Polyclonal antibody (26712-1-AP)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HEK-293T, cellules HepG2, lait humain, tissu cérébral de rat, tissu splénique de souris, tissu testiculaire de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain, human intrahepatic cholangiocarcinoma tissue; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

26712-1-AP cible NUCB2/nesfatin-1 dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NUCB2/nesfatin-1 Protéine recombinante Ag25152
• Nom complet: nucleobindin 2
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 40-50 kDa
• Numéro d’acquisition GenBank: NM_005013
• Symbole du gène: NUCB2
• Identification du gène (NCBI): 4925
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NucB2, an anorexigenic molecule, is expressed mainly in the hypothalamus, particularly in the supraoptic nucleus (SON) and the paraventricular nucleus (PVN). NucB2 is also expressed in the subfornical organ (SFO). Because the SON and PVN are involved in body fluid regulation, NucB2 may be involved in dehydration-induced anorexia. NUCB2 is composed of a signal peptide of 24 amino acids in the N-terminal region and a protin structure containing 396 amino acids. Nesfatin-1 is an 82-amino-acid peptide hormone cleaved from nucleobindin2 (NUCB2).

Publications

Species	Application	Title
human	WB	Proteomics; PMID: 29901268; Authors - Wang Li
human	WB	J Transl Med; NUCB2/Nesfatin-1 drives breast cancer metastasis through the up-regulation of cholesterol synthesis via the mTORC1 pathway; Authors - Siyi Ning
rat,mouse	WB,IF	Cell Commun Signal; Nesfatin-1 enhances vascular smooth muscle calcification through facilitating BMP-2 osteogenic signaling; Authors - Xue-Xue Zhu ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Guorong (Verified Customer) (11-22-2022)

Clear and specific band

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: ARPE-19

NUCB2/nesfatin-1 Antibody Western Blot validation (1:500 dilution) in ARPE-19 (Cat no:26712-1-AP)

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