Anticorps Polyclonal de lapin anti-Occludin

• Phare
• Validé par KD/KO

Occludin Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (4)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

367

Avis (5)

N° de cat : 13409-1-AP

Synonymes

occludin, OCLN

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Galerie de données de validation

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  WB analysis using 13409-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13409-1-AP (Occludin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 13409-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13409-1-AP (Occludin Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 13409-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13409-1-AP (Occludin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 13409-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13409-1-AP (Occludin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 13409-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 13409-1-AP (OCLN antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 13409-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13409-1-AP (OCLN antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 13409-1-AP

  IP Result of anti-Occludin (IP:13409-1-AP, 3ug; Detection:13409-1-AP 1:500) with mouse liver tissue lysate 6400ug.

• 

  IHC staining of human breast cancer using 13409-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13409-1-AP (Occludin Antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 13409-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13409-1-AP (Occludin Antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 13409-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13409-1-AP (Occludin Antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 13409-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13409-1-AP (Occludin Antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 13409-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Occludin antibody (13409-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of A431 using 13409-1-AP

  1X10^6 A431 cells were stained with 0.2 ug Anti-Human Occludin (13409-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), or stained with 0.2 ug isotype control antibody and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (black). Cells were fixed with 90% MeOH.

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Applications testées

• Résultats positifs en WB: cellules A431, tissu de côlon de souris, tissu hépatique de rat, tissu hépatique de souris, tissu hépatique humain, tissu rénal de rat, tissu rénal de souris, tissu rénal humain
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7,
• Résultats positifs en cytométrie: cellules A431

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:800
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 298 publications below
• IHC: See 49 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Informations sur le produit

13409-1-AP cible Occludin dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, canin, Humain, porc, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Occludin Protéine recombinante Ag4057
• Nom complet: occludin
• Masse moléculaire calculée: 522 aa, 59 kDa
• Poids moléculaire observé: 59 kDa
• Numéro d’acquisition GenBank: BC029886
• Symbole du gène: OCLN
• Identification du gène (NCBI): 4950
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms, owing to modifications at the posttranscriptional and posttranslational levels, the monomeric occludin migrates as 53-65 kDa on SDS-PAGE (PMID: 22083955; 19457074).

Publications

Species	Application	Title
mouse	IHC	Cell Host Microbe; Gut microbiome dysbiosis contributes to abdominal aortic aneurysm by promoting neutrophil extracellular trap formation; Authors - Zhenyu Tian
human	WB	Hepatology; Basolateral CD147 induces hepatocyte polarity loss by E-cadherin ubiquitination and degradation in hepatocellular carcinoma progress.; Authors - Meng Lu
mouse	WB	J Exp Med; Brain endothelial TAK1 and NEMO safeguard the neurovascular unit.; Authors - Dirk A Ridder
human	WB	Nat Commun; Daxx inhibits hypoxia-induced lung cancer cell metastasis by suppressing the HIF-1α/HDAC1/Slug axis.; Authors - Ching-Wen Lin
human	WB	Mater Today Bio; ROS-responsive nanoparticles for oral delivery of luteolin and targeted therapy of ulcerative colitis by regulating pathological microenvironment.; Authors - Chen Tan
mouse		EMBO Mol Med; Deficiency in intestinal epithelial O-GlcNAcylation predisposes to gut inflammation.; Authors - Ming Zhao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tom (Verified Customer) (02-03-2023)

1:5000 on murine intestinal protein extract for 2h in TBST

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Mouse intestines

FH

Wu (Verified Customer) (12-09-2021)

The antibody gives a good detection of Occludin

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: gut

Occludin Antibody Immunofluorescence validation (1:100 dilution) in gut (Cat no:13409-1-AP)

FH

Tom (Verified Customer) (09-21-2021)

Gave decent results with IHC at a concentration of 1:300-1:400 for mouse intestinal tissue

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:300-1:400
• Cell Tissue Type: Mouse intestinal tissue

FH

Amy (Verified Customer) (05-22-2019)

I approached Proteintech for guidance with regards to dilutions of this antibody, and with their help I optimized the protocol in good time. Very helpful!

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse Colon

FH

JIMMY (Verified Customer) (03-26-2019)

This antibody offers a good detection of Occludin on mouse brain endothelial cell line, bEnd3 cells, through IHC.

• Applications: Immunofluorescence,

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