Anticorps Monoclonal anti-ODC1

• Phare
• Validé par KD/KO

ODC1 Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain et plus (1)

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

2A1E3

N° de cat : 67336-1-Ig

Synonymes

2A1E3, EC:4.1.1.17, ODC

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Galerie de données de validation

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  WB analysis of MCF-7 using 67336-1-Ig

  WB result of ODC1 antibody (67336-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ODC1 transfected MCF-7 cells.

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  WB analysis of MCF-7 using 67336-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67336-1-Ig (ODC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 67336-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67336-1-Ig (ODC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HUVEC using 67336-1-Ig

  HUVEC cells were subjected to SDS PAGE followed by western blot with 67336-1-Ig (ODC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 67336-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using ODC1 antibody (67336-1-Ig, Clone: 2A1E3 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules HUVEC, tissu placentaire humain
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

67336-1-Ig cible ODC1 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ODC1 Protéine recombinante Ag10927
• Nom complet: ornithine decarboxylase 1
• Masse moléculaire calculée: 461 aa, 51 kDa
• Poids moléculaire observé: 51 kDa
• Numéro d’acquisition GenBank: BC025296
• Symbole du gène: ODC1
• Identification du gène (NCBI): 4953
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ornithine decarboxylase (ODC) is also named as ODC1 and belongs to the Orn/Lys/Arg decarboxylase class-II family. It catalyzes the conversion of ornithine to putrescine, the first step and a major site of regulation of polyamine biosynthesis. The level of ODC is known to be controlled at several sites, namely transcription, translation, and enzyme degradation. Polyamines can stimulate the degradation of ODC as a type of negative feedback control (PMID:8486633). This protein can be phosphorylated in vivo (PMID:8798774). ODC1 can form a homodimer and only the dimer is catalytically active, as the active sites are constructed of residues from both monomers (PMID: 10623504). The molecular mass of ODC1 is 51 kDa, and the homodimer is 106 kDa.

Publications

Species	Application	Title
mouse	WB	J Pharm Biomed Anal; Combined untargeted metabolomics and network pharmacology approaches to reveal the therapeutic role of withanolide B in psoriasis; Authors - Tingting Li

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