Anticorps Polyclonal de lapin anti-OGDH

• Phare
• Validé par KD/KO

OGDH Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

56

N° de cat : 15212-1-AP

Synonymes

AKGDH, E1k, OGDC, OGDC E1, OGDH

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Galerie de données de validation

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  WB analysis using 15212-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15212-1-AP (OGDH antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15212-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15212-1-AP (OGDH antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of ROS1728 using 15212-1-AP

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 15212-1-AP (OGDH Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 15212-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15212-1-AP (OGDH antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 15212-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15212-1-AP (OGDH antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of rat lung using 15212-1-AP

  rat lung tissue were subjected to SDS PAGE followed by western blot with 15212-1-AP (OGDH Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 15212-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15212-1-AP (OGDH antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 15212-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15212-1-AP (OGDH antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15212-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells stained for OGDH rabbit pAb (15212-1-AP) at dilution of 1:50 and alpha Tubulin mouse mAb (66031-1-Ig) at dilution of 1:100, further stained with Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 15212-1-AP, and Alexa Fluor 594-conjugated AffiniPure Goat Anti-mouse IgG (H+L) for 66031-1-Ig. .

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  IF Staining of A549 using 15212-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using OGDH antibody (15212-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 15212-1-AP

  Immunofluorescent analysis of Hela cells, using OGDH antibody 15212-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, cellules A549, cellules ROS1728, tissu cardiaque de rat, tissu hépatique de souris, tissu pancréatique de souris, tissu pulmonaire de souris
• Résultats positifs en IHC: tissu de cancer du poumon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa, cellules A549

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:10000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 53 publications below
• IHC: See 5 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Informations sur le produit

15212-1-AP cible OGDH dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, Lapin, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: OGDH Protéine recombinante Ag7368
• Nom complet: oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide)
• Masse moléculaire calculée: 116 kDa
• Poids moléculaire observé: 116 kDa
• Numéro d’acquisition GenBank: BC004964
• Symbole du gène: OGDH
• Identification du gène (NCBI): 4967
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

OGDH(2-oxoglutarate dehydrogenase, mitochondrial) is also named as OGDC-E1and belongs to the alpha-ketoglutarate dehydrogenase family. It is one of the three enzymatic components of 2-oxoglutarate dehydrogenase complex,which catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. This protein has 3 isoforms produced by alternative splicing. This antibody is immunized with the C-terminal and can recognize the isoform 1 and 2 with the MW of 116 kDa.

Publications

Species	Application	Title
human,mouse	WB,IHC	Nature; α-Ketoglutarate links p53 to cell fate during tumour suppression.; Authors - John P Morris ; KD Validated
	WB	Cell Metab; Rewiring of Glutamine Metabolism Is a Bioenergetic Adaptation of Human Cells with Mitochondrial DNA Mutations.; Authors - Qiuying Chen
human,mouse	WB	Cell Metab; An Upstream Open Reading Frame in Phosphatase and Tensin Homolog Encodes a Circuit Breaker of Lactate Metabolism.; Authors - Nunu Huang
human	WB	Cell Res; The metabolite α-KG induces GSDMC-dependent pyroptosis through death receptor 6-activated caspase-8.; Authors - Jia-Yuan Zhang
mouse	IF	Nat Neurosci; A glycolytic shift in Schwann cells supports injured axons.; Authors - Elisabetta Babetto
human	WB	J Hepatol; OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism.; Authors - Weiqi Dai