Anticorps Polyclonal de lapin anti-OGDHL

• Phare
• Validé par KD/KO

OGDHL Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(14)

N° de cat : 17110-1-AP

Synonymes

KIAA1290, OGDC E1 like, OGDHL

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Galerie de données de validation

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  WB analysis using 17110-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17110-1-AP (OGDHL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 17110-1-AP

  IP result of anti-OGDHL (IP:17110-1-AP, 4ug; Detection:17110-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of human liver using 17110-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17110-1-AP (OGDHL antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 17110-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17110-1-AP (OGDHL antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat, tissu rénal de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu hépatique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 11 publications below
• IHC: See 5 publications below
• IF: See 2 publications below
• FC: See 1 publications below

Informations sur le produit

17110-1-AP cible OGDHL dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: OGDHL Protéine recombinante Ag10794
• Nom complet: oxoglutarate dehydrogenase-like
• Masse moléculaire calculée: 1010 aa, 115 kDa
• Poids moléculaire observé: 115 kDa
• Numéro d’acquisition GenBank: BC026320
• Symbole du gène: OGDHL
• Identification du gène (NCBI): 55753
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

OGDHL(oxoglutarate dehydrogenase-like) is also named as KGDE1KL, FLJ10851, KIAA1290 and belongs to the alpha-ketoglutarate dehydrogenase family. OGDHL is one of the rate-limiting component of the multienzyme OGDH complex (OGDHC) whose malfunction is associated with neuro-degeneration and it is localized only in the mitochondrial fraction and not in the cytosolic or nuclear fractions(PMID:23152800). OGDHL negatively regulates cell proliferation by inducing apoptosis in cervical cancer cells. It may thus participate in brain-specific control of 2-oxoglutarate distribution between energy production and synthesis of the neurotransmitter glutamate(PMID:18783430). It has 3 isoforms(114 kDa, 108 kDa, 91 kDa) produced by alternative splicing.

Publications

Species	Application	Title
human	WB	J Hepatol; OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism.; Authors - Weiqi Dai ; KD Validated
human	WB	Nat Commun; A reversible state of hypometabolism in a human cellular model of sporadic Parkinson's disease; Authors - Sebastian Schmidt
human	WB	Am J Hum Genet; Bi-allelic variants in OGDHL cause a neurodevelopmental spectrum disease featuring epilepsy, hearing loss, visual impairment, and ataxia.; Authors - Zheng Yie Yap
mouse	WB	Cell Commun Signal; REST contributes to renal fibrosis through inducing mitochondrial energy metabolism imbalance in tubular epithelial cells; Authors - Yingxian Yang
mouse	WB	Front Cell Dev Biol; Cardioprotective Role of SIRT5 in Response to Acute Ischemia Through a Novel Liver-Cardiac Crosstalk Mechanism.; Authors - Boda Zhou
rat	WB	Transl Psychiatry; Hippocampal proteomic changes of susceptibility and resilience to depression or anxiety in a rat model of chronic mild stress.; Authors - Min Tang

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