Anticorps Polyclonal de lapin anti-Osteoglycin

Osteoglycin Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

5

Avis (1)

N° de cat : 12755-1-AP

Synonymes

Mimecan, OG, OGN, OIF, Osteoglycin, Osteoinductive factor, SLRR3A

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 12755-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12755-1-AP (Osteoglycin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 12755-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12755-1-AP (Osteoglycin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12755-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12755-1-AP (Osteoglycin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung using 12755-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 12755-1-AP (Osteoglycin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 12755-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 12755-1-AP (Osteoglycin antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 12755-1-AP

  Immunofluorescent analysis of HepG2 cells, using OGN antibody 12755-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, cellules HeLa, tissu hépatique humain
• Résultats positifs en IHC: tissu pulmonaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

12755-1-AP cible Osteoglycin dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Osteoglycin Protéine recombinante Ag3484
• Nom complet: osteoglycin
• Masse moléculaire calculée: 298 aa, 34 kDa
• Poids moléculaire observé: 34-40 kDa
• Numéro d’acquisition GenBank: BC037273
• Symbole du gène: OGN
• Identification du gène (NCBI): 4969
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

This gene encodes a member of the small leucine-rich proteoglycan (SLRP) family of proteins. The encoded protein induces ectopic bone formation in conjunction with transforming growth factor beta and may regulate osteoblast differentiation. High expression of the encoded protein may be associated with elevated heart left ventricular mass. Alternative splicing results in multiple transcript variants which forms proteins with different molecular weight.

Publications

Species	Application	Title
human	IHC, IF	FASEB J; The "Matrisome" reveals the characterization of skin keloid microenvironment.; Authors - Shikun Zhang
mouse	IHC	J Inflamm Res; The m6A/m1A/m5C-Related Methylation Modification Patterns and Immune Landscapes in Rheumatoid Arthritis and Osteoarthritis Revealed by Microarray and Single-Cell Transcriptome; Authors - Haishi Zheng
mouse	IHC	Sci Rep; Proteomic profiling of concurrently isolated primary microvascular endothelial cells, pericytes, and vascular smooth muscle cells from adult mouse heart.; Authors - Zhiping Cao
human	WB	Onco Targets Ther; Osteoglycin (OGN) Inhibits Cell Proliferation and Invasiveness in Breast Cancer via PI3K/Akt/mTOR Signaling Pathway.; Authors - Tao Xu
human	WB	Front Immunol; Identification of nine signature proteins involved in periodontitis by integrated analysis of TMT proteomics and transcriptomics; Authors - Wei Liu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Huai-Chin (Verified Customer) (10-24-2022)

Multiple bands in Western blot. There are two dominant bands at ~40KD and ~55KD. The one ~40KD maybe the band of interest.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: used on both mouse and human cell lines