Anticorps Polyclonal de lapin anti-OLR1

• Phare
• Validé par KD/KO

OLR1 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

38

N° de cat : 11837-1-AP

Synonymes

CLEC8A, hLOX 1, Lectin like oxLDL receptor 1, LOX 1, LOX1, OLR1, Ox LDL receptor 1, SCARE1

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Galerie de données de validation

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  WB analysis using 11837-1-AP

  bEnd.3 cells were subjected to SDS PAGE followed by western blot with 11837-1-AP (OLR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 11837-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11837-1-AP (OLR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of L02 using 11837-1-AP

  IP Result of anti-OLR1 (IP:11837-1-AP, 4ug; Detection:11837-1-AP 1:800) with L02 cells lysate 1800ug.

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  IHC staining of human heart using 11837-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11837-1-AP (OLR1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 11837-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11837-1-AP (OLR1 Antibody) at dilution of 1:200 (under 40x lens).

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  FC experiment of HUVEC using 11837-1-AP

  1X10^6 HUVEC cells were stained with 0.2ug OLR1 antibody (11837-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: cellules bEnd.3, tissu hépatique humain
• Résultats positifs en IP: cellules L02
• Résultats positifs en IHC: tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules HUVEC

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 27 publications below
• IHC: See 10 publications below
• IF: See 6 publications below
• CoIP: See 1 publications below

Informations sur le produit

11837-1-AP cible OLR1 dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: OLR1 Protéine recombinante Ag2437
• Nom complet: oxidized low density lipoprotein (lectin-like) receptor 1
• Masse moléculaire calculée: 273 aa, 31 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC022295
• Symbole du gène: OLR1
• Identification du gène (NCBI): 4973
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

OLR1/LOX-1 acts as a receptor, in the form of homodimer, that mediates the recognition, internalization, and degradation of oxidatively modified low density lipoprotein (oxLDL) (PMID: 9052782, 15695803). ORL1 is predominantly expressed in endothelial cells and vascular-rich organs such as the placenta, lung, liver, and brain (PMID: 9828121). OLR1 is a protein containing 273 amino acids with a calculated molecular mass of 31 kDa but an apparent molecular mass of 50 kDa, which may result from the glycosylation of four potential N-linked glycosylation sites located at the C-terminal domain (PMID: 9052782).

Publications

Species	Application	Title
mouse	WB	Free Radic Biol Med; Up-regulation of thioredoxin system by puerarin inhibits lipid uptake in macrophages; Authors - Wenchao Li,
human	WB	Cancer Lett; Up-regulation of OLR1 expression by TBC1D3 through activation of TNFα/NF-κB pathway promotes the migration of human breast cancer cells.; Authors - Bei Wang ; KD Validated
human	WB	Metabolism; Deacetylation of Caveolin-1 by Sirt6 induces autophagy and retards high glucose-stimulated LDL transcytosis and atherosclerosis formation.; Authors - Ying Zhao
human	WB	Sci Rep; Lectin-like oxidized low-density lipoprotein receptor-1 facilitates metastasis of gastric cancer through driving epithelial-mesenchymal transition and PI3K/Akt/GSK3β activation.; Authors - Can Li ; KD Validated
mouse	IHC	Int J Cancer; The oxidized-LDL/LOX-1 axis in tumor endothelial cells enhances metastasis by recruiting neutrophils and cancer cells.; Authors - Takuya Tsumita
human	WB	Mol Cancer Res; OLR1 Promotes Pancreatic Cancer Metastasis via Increased c-Myc Expression and Transcription of HMGA2.; Authors - Gang Yang ; KD Validated

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