Anticorps Monoclonal anti-OMA1

OMA1 Monoclonal Antibody for WB,ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB,ELISA

Conjugaison

Non conjugué

CloneNo.

1H4D12

N° de cat : 67449-1-Ig

Synonymes

2010001O09Rik, DAB1, FLJ33782, MPRP 1, MPRP1, OMA1, YKR087C, ZMPOMA1

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 67449-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67449-1-Ig (OMA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67449-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67449-1-Ig (OMA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67449-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67449-1-Ig (OMA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of LNCaP using 67449-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67449-1-Ig (OMA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 67449-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67449-1-Ig (OMA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, cellules HEK-293, cellules HeLa, cellules Jurkat, cellules LNCaP

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67449-1-Ig cible OMA1 dans les applications de WB,ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: OMA1 Protéine recombinante Ag11201
• Nom complet: OMA1 homolog, zinc metallopeptidase (S. cerevisiae)
• Masse moléculaire calculée: 524 aa, 60 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC012915
• Symbole du gène: OMA1
• Identification du gène (NCBI): 115209
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

OMA1(Overlapping with the m-AAA protease 1 homolog) is also named as MPRP1(Metalloprotease-related protein 1) and belongs to the peptidase M48 family.It is a zinc metalloprotease that spans the inner mitochondrial membrane with a number of predicted membrane spanning domains and the 60 kDa form of OMA1 rapidly accumulated concomitantly with the cleavage of all OPA1 variants,and the 40 kDa form of OMA1 may be the inactive form, with the 60 kDa form being the active enzyme(PMID:20334834)