Anticorps Polyclonal de lapin anti-ONECUT1

ONECUT1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 25137-1-AP

Synonymes

Hepatocyte nuclear factor 6, HNF 6, HNF6, HNF6A, One cut domain family member 1, one cut homeobox 1, ONECUT1

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Galerie de données de validation

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  WB analysis of mouse liver using 25137-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 25137-1-AP (ONECUT1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 25137-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25137-1-AP (ONECUT1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver cancer using 25137-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25137-1-AP (ONECUT1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 25137-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25137-1-AP (ONECUT1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 25137-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25137-1-AP (ONECUT1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 25137-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25137-1-AP (ONECUT1 Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver cancer using 25137-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25137-1-AP (ONECUT1 Antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver cancer using 25137-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25137-1-AP (ONECUT1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 25137-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25137-1-AP (ONECUT1 Antibody) at dilution of 1:50 (under 10x lens).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IF: See 2 publications below

Informations sur le produit

25137-1-AP cible ONECUT1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ONECUT1 Protéine recombinante Ag18250
• Nom complet: one cut homeobox 1
• Masse moléculaire calculée: 465 aa, 51 kDa
• Poids moléculaire observé: 51-55 kDa
• Numéro d’acquisition GenBank: BC140830
• Symbole du gène: ONECUT1
• Identification du gène (NCBI): 3175
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ONECUT1, also named as Hepatocyte nuclear factor 6 (HNF6), is a 465 amino acid protein, which contains one CUT DNA-binding domain and one homeobox DNA-binding domain. ONECUT1 localizes in the nucleus and belongs to the CUT homeobox family. ONECUT1 as a transcription activator is involved in the some liver genes transcription. ONECUT1 is highly expressed in liver and lower in testis, skin.

Publications

Species	Application	Title
mouse	WB	J Clin Invest; REVERBa couples the circadian clock to hepatic glucocorticoid action.; Authors - Giorgio Caratti
human	WB	J Virol; Inhibition of Hepatitis B Virus Gene Expression and Replication by Hepatocyte Nuclear Factor 6.; Authors - Ruidong Hao
human	WB	Virol Sin; Psoralen inhibits hepatitis B viral replication by down-regulating the host transcriptional machinery of viral promoters; Authors - Xinna Ma
mouse	IF	bioRxiv; Enhanced Plasmid-Based Transcriptional Activation in Developing Mouse Photoreceptors; Authors - Brendon M Patierno
zebrafish	WB	Cells; The Zebrafish Retina and the Evolution of the Onecut-Mediated Pathway in Cell Type Differentiation; Authors - Quirino Attilio Vassalli
human	IF	Invest Ophthalmol Vis Sci; Enhanced Transcriptional Activation in Developing Mouse Photoreceptors; Authors - Brendon M Patierno

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