Anticorps Polyclonal de lapin anti-ONECUT2

• Phare
• Validé par KD/KO

ONECUT2 Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IHC, ChIP, Cell treatment, ELISA

Conjugaison

Non conjugué

Publications

12

N° de cat : 21916-1-AP

Synonymes

HNF 6 beta, HNF6B, OC 2, OC2, One cut domain family member 2, one cut homeobox 2, ONECUT2, Transcription factor ONECUT 2

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Galerie de données de validation

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  WB analysis using 21916-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21916-1-AP (ONECUT2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 21916-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21916-1-AP (ONECUT2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skin using 21916-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 21916-1-AP (ONECUT2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 21916-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21916-1-AP (ONECUT2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 21916-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 21916-1-AP (ONECUT2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 21916-1-AP

  IP Result of anti-ONECUT2 (IP:21916-1-AP, 4ug; Detection:21916-1-AP 1:300) with HEK-293 cells lysate 3200ug.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules HEK-293, cellules HepG2, cellules Jurkat, tissu cutané de souris
• Résultats positifs en IP: cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 2 publications below
• ChIP: See 3 publications below

Informations sur le produit

21916-1-AP cible ONECUT2 dans les applications de WB, IP, IHC, ChIP, Cell treatment, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ONECUT2 Protéine recombinante Ag16162
• Nom complet: one cut homeobox 2
• Masse moléculaire calculée: 504 aa, 54 kDa
• Poids moléculaire observé: 54-60 kDa
• Numéro d’acquisition GenBank: BC108925
• Symbole du gène: ONECUT2
• Identification du gène (NCBI): 9480
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ONECUT2, also named as One cut domain family member 2 or Hepatocyte nuclear factor 6-beta, is a 504 amino acid protein, which contains one CUT DNA-binding domain and one homeobox DNA-binding domain. ONECUT2 belongs to the CUT homeobox family and activates the transcription of a number of liver genes such as HNF3B. ONECUT2 is required for liver differnetiation and metabolism. ONECUT2 and ONECUT3 play redundant roles with ONECUT1 in pancreas development.

Publications

Species	Application	Title
human	WB	Cancer Cell; Temporal evolution reveals bifurcated lineages in aggressive neuroendocrine small cell prostate cancer trans-differentiation; Authors - Chia-Chun Chen
human	WB	Nat Med; ONECUT2 is a targetable master regulator of lethal prostate cancer that suppresses the androgen axis.; Authors - Mirja Rotinen
human	IHC	Cancer Cell; Epigenomic State Transitions Characterize Tumor Progression in Mouse Lung Adenocarcinoma.; Authors - Lindsay M LaFave
human	WB	Sci Adv; Hypoxia-mediated stabilization of HIF1A in prostatic intraepithelial neoplasia promotes cell plasticity and malignant progression.; Authors - Mohamed A Abu El Maaty
human	WB	Proc Natl Acad Sci U S A; Addiction to Golgi-resident PI4P synthesis in chromosome 1q21.3-amplified lung adenocarcinoma cells.; Authors - Lei Shi ; KD Validated
human	WB,ChIP	Cell Death Dis; ONECUT2 facilitates hepatocellular carcinoma metastasis by transcriptionally upregulating FGF2 and ACLY; Authors - Danfei Liu ; KD Validated