Anticorps Polyclonal de lapin anti-OPA3

• Phare
• Validé par KD/KO

OPA3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 15638-1-AP

Synonymes

MGA3, OPA3, Optic atrophy 3 protein

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Galerie de données de validation

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  WB analysis using 15638-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15638-1-AP (OPA3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15638-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15638-1-AP (OPA3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 15638-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 15638-1-AP (OPA3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 15638-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 15638-1-AP (OPA3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15638-1-AP

  IP result of anti-OPA3 (IP:15638-1-AP, 4ug; Detection:15638-1-AP 1:1000) with HeLa cells lysate 1240 ug.

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  IHC staining of human liver using 15638-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15638-1-AP (OPA3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 15638-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15638-1-AP (OPA3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 15638-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 15638-1-AP (OPA3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 15638-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 15638-1-AP (OPA3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15638-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OPA3 antibody (15638-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293T, cellules HeLa, cellules Jurkat, tissu de thymus de souris, tissu rénal humain
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu hépatique humain, tissu de côlon de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 3 publications below
• IP: See 1 publications below

Informations sur le produit

15638-1-AP cible OPA3 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: OPA3 Protéine recombinante Ag8103
• Nom complet: optic atrophy 3 (autosomal recessive, with chorea and spastic paraplegia)
• Masse moléculaire calculée: 179 aa, 20 kDa
• Poids moléculaire observé: 20 kDa
• Numéro d’acquisition GenBank: BC005059
• Symbole du gène: OPA3
• Identification du gène (NCBI): 80207
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The OPA3 cDNA encodes a deduced 179-amino acid protein. Northern blot analysis demonstrated a primary transcript of approximately 5.0 kb that was ubiquitously expressed, most prominently in skeletal muscle and kidney. Mutations in this gene have been shown to result in 3-methylglutaconic aciduria type III and autosomal dominant optic atrophy and cataract.

Publications

Species	Application	Title
mouse	IHC	Hum Mol Genet; Disrupted mitochondrial function in the Opa3L122P mouse model for Costeff Syndrome impairs skeletal integrity.; Authors - Alice E Navein
mouse,rat	WB,IP	Cell Signal; Hydrogen sulfide alleviates mitochondrial damage and ferroptosis by regulating OPA3-NFS1 axis in doxorubicin-induced cardiotoxicity; Authors - Yifan Wang
human,mouse	WB,IHC	Invest Ophthalmol Vis Sci; Mitochondrial localization and ocular expression of mutant Opa3 in a mouse model of 3-methylglutaconicaciduria type III.; Authors - Powell Kate A KA
bovine	WB	Genomics; A nonsense mutation in the optic atrophy 3 gene (OPA3) causes dilated cardiomyopathy in Red Holstein cattle.; Authors - Owczarek-Lipska Marta M
human	WB	Bioengineered; MYB proto-oncogene like 2 promotes hepatocellular carcinoma growth and glycolysis via binding to the Optic atrophy 3 promoter and activating its expression.; Authors - Miao Liu ; KD Validated
human	IHC	Cancers (Basel); Oncogenic K-ras Induces Mitochondrial OPA3 Expression to Promote Energy Metabolism in Pancreatic Cancer Cells.; Authors - Ning Meng