Anticorps Monoclonal anti-ORAI1

ORAI1 Monoclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, rat, souris et plus (2)

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

18

CloneNo.

3C12F9

Avis (1)

N° de cat : 66223-1-Ig

Synonymes

ORAI1

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Galerie de données de validation

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  WB analysis using 66223-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66223-1-Ig (ORAI1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66223-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66223-1-Ig (ORAI1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 66223-1-Ig

  A375 cells were subjected to SDS PAGE followed by western blot with 66223-1-Ig (ORAI1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of Jurkat using 66223-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ORAI1 antibody (66223-1-Ig, Clone: 3C12F9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules 4T1, cellules A375, cellules Daudi, cellules Jurkat, cellules K-562, cellules Raji, cellules Ramos, cellules RAW 264.7, tissu de thymus de porc, tissu splénique de porc, tissu splénique de rat
• Résultats positifs en IF: cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:30000
• Immunofluorescence (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 17 publications below
• IF: See 5 publications below

Informations sur le produit

66223-1-Ig cible ORAI1 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, bovin, Humain, singe, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ORAI1 Protéine recombinante Ag18050
• Nom complet: ORAI calcium release-activated calcium modulator 1
• Masse moléculaire calculée: 301 aa, 33 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC015369
• Symbole du gène: ORAI1
• Identification du gène (NCBI): 84876
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Store-operated calcium channels (SOCs) play essential functions in various physiological processes. Orai1 (also named CRACM1) is a critical molecular component of SOCs. Orai1 protein is a 32-55 kDa plasma membrane protein with 4 transmembrane domains. Human patients with a severe combinaed immunodeficiency (SCID) syndrome possess point mutations in Orai1 gene.

Publications

Species	Application	Title
rat	WB	Cell Death Dis; Pathological convergence of APP and SNCA deficiency in hippocampal degeneration of young rats; Authors - Yajie Wang
human	WB,IF	Oncogenesis; Blockage of Orai1-Nucleolin interaction meditated calcium influx attenuates breast cancer cells growth; Authors - Chunming Gu
human	WB	Int J Mol Sci; Neuroprotective Effect of Antiapoptotic URG7 Protein on Human Neuroblastoma Cell Line SH-SY5Y; Authors - Ilaria Nigro
mouse	WB	Front Cell Dev Biol; Transmembrane Protein Ttyh1 Maintains the Quiescence of Neural Stem Cells Through Ca2+/NFATc3 Signaling.; Authors - Yuan Cao
mouse	WB	J Innate Immun; Mannan-Binding Lectin Reduces Epithelial-Mesenchymal Transition in Pulmonary Fibrosis via Inactivating the Store-Operated Calcium Entry Machinery.; Authors - Yunzhi Liu
mouse	WB	Cell Calcium; Mannan-binding lectin deficiency augments hepatic endoplasmic reticulum stress through IP3R-controlled calcium release.; Authors - Yu Chen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Udesh (Verified Customer) (01-19-2023)

The Ab worked well for WB. 20ug protein loaded. Probed overnight at 4 deg.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000 in 5% NFDM

ORAI1 Antibody Western Blot validation (1:2000 in 5% NFDM dilution) in (Cat no:66223-1-Ig)