Anticorps Polyclonal de lapin anti-OSBPL2

• Phare
• Validé par KD/KO

OSBPL2 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF-P, IP, ChIP, ELISA

Conjugaison

Non conjugué

Publications(11)

Avis (1)

N° de cat : 14751-1-AP

Synonymes

OSBP-related protein 2, OSBP related protein 2, ORP-2, ORP2, ORP 2

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Galerie de données de validation

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  WB analysis using 14751-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14751-1-AP (OSBPL2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 14751-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14751-1-AP (OSBPL2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14751-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14751-1-AP (OSBPL2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HuH-7 using 14751-1-AP

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 14751-1-AP (OSBPL2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 14751-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14751-1-AP (OSBPL2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14751-1-AP

  IP result of anti-OSBPL2 (IP:14751-1-AP, 4ug; Detection:14751-1-AP 1:500) with HeLa cells lysate 880ug.

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  IHC staining of human ovary cancer using 14751-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 14751-1-AP (OSBPL2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 14751-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using OSBPL2 antibody (14751-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules HepG2, cellules HuH-7, tissu cérébral de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: human ovary cancer tissue, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IF: See 5 publications below
• ChIP: See 1 publications below

Informations sur le produit

14751-1-AP cible OSBPL2 dans les applications de WB, IHC, IF-P, IP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: OSBPL2 Protéine recombinante Ag6496
• Nom complet: oxysterol binding protein-like 2
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 51 kDa, 56 kDa
• Numéro d’acquisition GenBank: BC000296
• Symbole du gène: OSBPL2
• Identification du gène (NCBI): 9885
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Protein Cell; ORP8 acts as a lipophagy receptor to mediate lipid droplet turnover; Authors - Maomao Pu
human	WB,IF	Autophagy; OSBPL2 mutations impair autophagy and lead to hearing loss, potentially remedied by rapamycin.; Authors - Young Ik Koh
mouse	IF	JCI Insight; Mutations in OSBPL2 cause hearing loss associated with primary cilia defects via Sonic Hedgehog signaling.; Authors - Hairong Shi
	WB	Genet Med; Identification of OSBPL2 as a novel candidate gene for progressive nonsyndromic hearing loss by whole-exome sequencing.; Authors - Guangqian Xing
human	IF,ChIP	Mol Cell Endocrinol; Oxysterol-related-binding-protein related Protein-2 (ORP2) regulates cortisol biosynthesis and cholesterol homeostasis.; Authors - Tamara Escajadillo ; KD Validated
human	WB	Exp Cell Res; OSBPL2 deficiency upregulate SQLE expression increasing intracellular cholesterol and cholesteryl ester by AMPK/SP1 and SREBF2 signalling pathway.; Authors - Cui Zhang ; KO Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Boyan (Verified Customer) (11-25-2024)

recognized a band at the expected molecular weight

• Applications: Western Blot

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