Anticorps Polyclonal de lapin anti-OSGEP

OSGEP Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 15033-1-AP

Synonymes

GCPL1, hOSGEP, KAE1, OSGEP, OSGEP1, PRSMG1

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Galerie de données de validation

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  WB analysis using 15033-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15033-1-AP (OSGEP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 15033-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 15033-1-AP (OSGEP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 15033-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 15033-1-AP (OSGEP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 15033-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15033-1-AP (OSGEP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 15033-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15033-1-AP (OSGEP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 15033-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15033-1-AP (OSGEP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 15033-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15033-1-AP (OSGEP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 15033-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15033-1-AP (OSGEP Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 15033-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15033-1-AP (OSGEP Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules PC-3, cellules Raji, tissu cardiaque humain, tissu de côlon de souris
• Résultats positifs en IHC: tissu rénal humain, tissu cardiaque humain, tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

15033-1-AP cible OSGEP dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: OSGEP Protéine recombinante Ag4308
• Nom complet: O-sialoglycoprotein endopeptidase
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: BC032310
• Symbole du gène: OSGEP
• Identification du gène (NCBI): 55644
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

OSGEP(O-sialoglycoprotein endopeptidase) is also named as hOSGEP, GCPL1 and belongs to the KAE1 / YgjD family. The deduced 335-amino acid OSGEP protein has a predicted molecular mass of 36.4 kDa. It is a protease whose target is O-linked sialic acid-containing glycoproteins, such as episialin(PMID:10859271). It is involved in the metabolism of glycated proteins as a glycoprotein-related chaperon protease(PMID: 20824107).

Publications

Species	Application	Title
human	WB	Nucleic Acids Res; Commonality and diversity in tRNA substrate recognition in t6A biogenesis by eukaryotic KEOPSs.; Authors - Jin-Tao Wang

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