Anticorps Polyclonal de lapin anti-OTUB2

• Phare
• Validé par KD/KO

OTUB2 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 12066-1-AP

Synonymes

Otubain-2, OTU2, OTU domain-containing ubiquitin aldehyde-binding protein 2, OTB2, EC:3.4.19.12

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Galerie de données de validation

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  WB analysis using 12066-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12066-1-AP (OTUB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 12066-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using OTUB2 antibody (12066-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, tissu testiculaire de rat
• Résultats positifs en IF/ICC: cellules U2OS,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

12066-1-AP cible OTUB2 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: OTUB2 Protéine recombinante Ag2703
• Nom complet: OTU domain, ubiquitin aldehyde binding 2
• Masse moléculaire calculée: 234 aa, 27 kDa
• Poids moléculaire observé: 27 kDa
• Numéro d’acquisition GenBank: BC009615
• Symbole du gène: OTUB2
• Identification du gène (NCBI): 78990
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

OTUB2, also known as C14orf137, belongs to the OTU family. OTUB2 is a functional cysteine protease with deubiquitinase activity. OTUB2 mediates the deubiquitination of substrate proteins, and the targets of substrate proteins can be diversified. Therefore, OTUB2 has a wide range of biological roles, including protecting against virus-induced activation of interferon regulatory factor 3 (IRF3) and NF-κB, promoting beta cell survival in human pancreatic islets, and supporting the DNA repair pathway (PMID: 30662561, 35309903). OTUB2 is widely expressed, with high expression levels in the brain. OTUB2 has 2 isoforms with molecular weights of 9 kDa and 27 kDa.

Publications

Species	Application	Title
human	WB,IF	Aging (Albany NY); The deubiquitinating enzymes-related signature predicts the prognosis and immunotherapy response in breast cancer; Authors - Youyuan Deng ; KD Validated

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