Anticorps Polyclonal de lapin anti-OTX2

• Phare
• Validé par KD/KO

OTX2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

26

Avis (1)

N° de cat : 13497-1-AP

Synonymes

Homeobox protein OTX2, MCOPS5, orthodenticle homeobox 2, Orthodenticle homolog 2, OTX2

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Galerie de données de validation

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  WB analysis using 13497-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13497-1-AP (OTX2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of Y79 using 13497-1-AP

  IP Result of anti-OTX2 (IP:13497-1-AP, 3ug; Detection:13497-1-AP 1:1500) with Y79 cells lysate 2400ug.

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  IP experiment of Y79 using 13497-1-AP

  IP Result of anti-OTX2 (IP:13497-1-AP, 4ug; Detection:13497-1-AP 1:2000) with Y79 cells lysate 2000ug.

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  IHC staining of human gliomas using 13497-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13497-1-AP (OTX2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human gliomas using 13497-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13497-1-AP (OTX2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of mouse embryo using 13497-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using 13497-1-AP (OTX2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse embryo using 13497-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using 13497-1-AP (OTX2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse embryo using 13497-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using 13497-1-AP (OTX2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of hESC cells using 13497-1-AP

  FC result of anti-OTX2 (13497-1-AP, 1:100) with hESC which spontaneous differentiation toward retinal lineage (RPE). (Black: control; Blue OTX2).

• 

  FC experiment of HepG2 using 13497-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human OTX2 (13497-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules Y79, tissu rétinien de rat, tissu rétinien de souris
• Résultats positifs en IP: cellules Y79
• Résultats positifs en IHC: tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu embryonnaire de souris,
• Résultats positifs en cytométrie: cellules HepG2, cellules SCEh

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 12 publications below
• IHC: See 6 publications below
• IF: See 8 publications below
• CoIP: See 1 publications below
• ChIP: See 6 publications below

Informations sur le produit

13497-1-AP cible OTX2 dans les applications de WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: OTX2 Protéine recombinante Ag4323
• Nom complet: orthodenticle homeobox 2
• Masse moléculaire calculée: 297 aa, 32 kDa
• Poids moléculaire observé: 31-35 kDa
• Numéro d’acquisition GenBank: BC032579
• Symbole du gène: OTX2
• Identification du gène (NCBI): 5015
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The orthodenticle homeobox 2, encoded by OTX2 gene, is a key transcription factor in developmental processes. In particular, it is required for the early specification of the brain and the embryonic development of sensory organs, including the, pineal gland, pituitary gland, inner part of the ear, eyes, and optic nerve. In later stages, it is important for maintaining intact retina and brain function. In addition, it acts as a transcriptional repressor and a gatekeeper of myogenic and neuronal differentiation in medulloblastoma cells. OTX2 binds to the MyoD1 core enhancer through its homeobox domain and the remarkable repressor activity exhibited by the homeobox domain renders OTX2 transcriptionally repressive

Publications

Species	Application	Title
human	WB	Nature; Unified rhombic lip origins of group 3 and group 4 medulloblastoma; Authors - Kyle S. Smith
mouse	IF	Nature; Reversing a model of Parkinson's disease with in situ converted nigral neurons.; Authors - Hao Qian
mouse	WB	Signal Transduct Target Ther; MDIG-mediated H3K9me3 demethylation upregulates Myc by activating OTX2 and facilitates liver regeneration; Authors - Jinpeng Du ; KD Validated
mouse	IF,ChIP	Science; Early life stress confers lifelong stress susceptibility in mice via ventral tegmental area OTX2.; Authors - Catherine J Peña
human	chip	Cancer Cell; Atypical Teratoid/Rhabdoid Tumors Are Comprised of Three Epigenetic Subgroups with Distinct Enhancer Landscapes.; Authors - Pascal D Johann
mouse	WB,ChIP	Cell Rep; Otx2 and Oct4 Drive Early Enhancer Activation during Embryonic Stem Cell Transition from Naive Pluripotency.; Authors - Shen-Hsi Yang ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

David (Verified Customer) (02-21-2022)

This antibody works very well for immunofluorescence on human brain.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1 to 200
• Cell Tissue Type: Human brain

OTX2 Antibody Immunofluorescence validation (1 to 200 dilution) in Human brain (Cat no:13497-1-AP)

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