Anticorps Monoclonal anti-Occludin

• Phare
• Validé par KD/KO

Occludin Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

72

CloneNo.

1D3C4

Avis (2)

N° de cat : 66378-1-Ig

Synonymes

Occludin, OCLN

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Galerie de données de validation

• 

  WB analysis using 66378-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66378-1-Ig (Occludin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66378-1-Ig

  WB result of Occludin antibody (66378-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-Occludin transfected Jurkat cells.

• 

  WB analysis of LNCaP using 66378-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 66378-1-Ig (Occludin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66378-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66378-1-Ig (Occludin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig colon using 66378-1-Ig

  pig colon tissue were subjected to SDS PAGE followed by western blot with 66378-1-Ig (Occludin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66378-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66378-1-Ig (Occludin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 66378-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66378-1-Ig (Occludin antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66378-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66378-1-Ig (Occludin antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 66378-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66378-1-Ig (Occludin antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 66378-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66378-1-Ig (Occludin antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 66378-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Occludin antibody (66378-1-Ig, Clone: 1D3C4 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of Caco-2 using 66378-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using Occludin antibody (66378-1-Ig, Clone: 1D3C4 ) at dilution of 1:1600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HUVEC, cellules 4T1, cellules HEK-293, cellules Jurkat, cellules LNCaP, cellules U2OS, tissu de côlon de porc, tissu de côlon de rat, tissu de côlon de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules Caco-2, cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:800-1:3200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 49 publications below
• IHC: See 10 publications below

Informations sur le produit

66378-1-Ig cible Occludin dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, bovin, Humain, porc, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Occludin Protéine recombinante Ag4057
• Nom complet: occludin
• Masse moléculaire calculée: 522 aa, 59 kDa
• Poids moléculaire observé: 59 kDa
• Numéro d’acquisition GenBank: BC029886
• Symbole du gène: OCLN
• Identification du gène (NCBI): 4950
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms, owing to modifications at the posttranscriptional and posttranslational levels, the monomeric occludin migrates as 53-65 kDa on SDS-PAGE (PMID: 22083955; 19457074).

Publications

Species	Application	Title
human		Circ Res; Endothelial BACE1 Impairs Cerebral Small Vessels via Tight Junctions and eNOS.; Authors - Haoyue Zhou
mouse	WB	Sci Adv; Astrocytic NDRG2-PPM1A interaction exacerbates blood-brain barrier disruption after subarachnoid hemorrhage; Authors - Dayun Feng
mouse	WB	Drug Des Devel Ther; Shensong Yangxin Capsule Reduces the Susceptibility of Arrhythmia in db/db Mice via Inhibiting the Inflammatory Response Induced by Endothelium Dysfunction; Authors - Jiehan Zhang
human,mouse		J Am Soc Nephrol; Close Association Between Altered Urine-urothelium Barrier and Tertiary Lymphoid Structure Formation in the Renal Pelvis During Nephritis.; Authors - Osamu Ichii
mouse	WB	J Mol Cell Biol; NRF1-mediated microglial activation triggers high-altitude cerebral edema.; Authors - Xueting Wang
mouse	IHC	Biomed Pharmacother; Bacterial butyrate mediates the anti-atherosclerotic effect of silybin; Authors - Hao-Ran Shen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Saba (Verified Customer) (07-25-2022)

The quality of the band is very good and you can easily find the specific band on your membrane.

• Applications: Western Blot

FH

Doriane (Verified Customer) (08-25-2019)

Satisfying antibody used for automated Western-blot Wes (Protein Simple)

• Applications: Western Blot, Other,
• Primary Antibody Dilution: Automated Western Blot Wes 1/400
• Cell Tissue Type: Caco-2/TC7 cell line

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