Anticorps Polyclonal de lapin anti-P21

• Phare
• Validé par KD/KO

P21 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (3)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

672

Avis (4)

N° de cat : 10355-1-AP

Synonymes

CAP20, CDK interacting protein 1, CDKN1, CDKN1A, CIP1, MDA 6, MDA6, P21, p21CIP1, PIC1, SDI1, WAF1

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Galerie de données de validation

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  WB analysis using 10355-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10355-1-AP (P21 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10355-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10355-1-AP (P21 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10355-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10355-1-AP (P21 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10355-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10355-1-AP (P21 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10355-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10355-1-AP (P21 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10355-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10355-1-AP (P21 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 10355-1-AP

  IP result of anti-P21(IP:10355-1-AP, 4ug; Detection:10355-1-AP 1:2000) with MCF-7 cells lysate 1880 ug.

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  IHC staining of human cervical cancer using 10355-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10355-1-AP (P21;CDKN1A Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 10355-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10355-1-AP (P21;CDKN1A Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 10355-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10355-1-AP (P21;CDKN1A antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 10355-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10355-1-AP (P21;CDKN1A antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 10355-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10355-1-AP (P21 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 10355-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10355-1-AP (P21 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 10355-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10355-1-AP (P21;CDKN1A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10355-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10355-1-AP (P21;CDKN1A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10355-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10355-1-AP (P21;CDKN1A antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10355-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10355-1-AP (P21;CDKN1A antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 10355-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10355-1-AP (P21;CDKN1A antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 10355-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10355-1-AP (P21;CDKN1A antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 10355-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 10355-1-AP (P21;CDKN1A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of myotome of a somite at E11.5 tissue using 10355-1-AP

  IF result of anti-p21 (CDKN1A, 10355-1-AP) in the myotome of a somite at E11.5 by Dr. Zalc A. and Dr. Relaix F. (Red = p21; Green=Pax3 from a GFP reporter; Blue=DAPI).

• 

  IF Staining of MCF-7 using 10355-1-AP

  Immunofluorescent analysis of MCF-7 cells using 10355-1-AP (P21;CDKN1A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of MCF-7 using 10355-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human P21 (10355-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules HUVEC, cellules HEK-293, cellules HeLa, cellules HepG2, cellules LNCaP, cellules MCF-7, cellules NCCIT, cellules THP-1, cellules U2OS
• Résultats positifs en IP: cellules MCF-7,
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, tissu de cancer de la thyroïde humain, tissu de cancer de l'estomac humain, tissu de cancer du côlon humain, tissu de cancer du sein humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7, tissu de myotome d'un somite à E11.5
• Résultats positifs en cytométrie: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:600
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 10 publications below
• WB: See 624 publications below
• IHC: See 54 publications below
• IF: See 38 publications below
• IP: See 2 publications below
• FC: See 1 publications below
• CoIP: See 2 publications below

Informations sur le produit

10355-1-AP cible P21 dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: canin, Humain, Lapin, porc
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: P21 Protéine recombinante Ag0368
• Nom complet: cyclin-dependent kinase inhibitor 1A (p21, Cip1)
• Masse moléculaire calculée: 18 kDa
• Poids moléculaire observé: 21 kDa
• Numéro d’acquisition GenBank: BC000275
• Symbole du gène: CDKN1A
• Identification du gène (NCBI): 1026
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CDKN1A (p21, CIP1, WAF1) is a cyclin-dependent kinase inhibitor. CDKN1A binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at the G1 phase. The expression of CDKN1A is induced by wild-type but not mutant p53 protein, through which CDKN1A mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. CDKN1A can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. CDKN1A was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported.

Publications

Species	Application	Title
human	WB,IHC	Gastroenterology; Intestinal PPARα Protects Against Colon Carcinogenesis via Regulation of Methyltransferases DNMT1 and PRMT6.; Authors - Yuhong Luo
human	WB	Sci Transl Med; Guanosine diphosphate-mannose suppresses homologous recombination repair and potentiates antitumor immunity in triple-negative breast cancer; Authors - Jia-Han Ding
human	WB	Nat Commun; URI alleviates tyrosine kinase inhibitors-induced ferroptosis by reprogramming lipid metabolism in p53 wild-type liver cancers; Authors - Zhiwen Ding
human	WB	Nat Commun; Phosphoglycerate dehydrogenase activates PKM2 to phosphorylate histone H3T11 and attenuate cellular senescence; Authors - Yinsheng Wu
human	WB	Nat Commun; Senescence-associated 13-HODE production promotes age-related liver steatosis by directly inhibiting catalase activity; Authors - Jinjie Duan
human	WB,IHC	Mol Cancer; Circular RNA hsa_circ_0004872 inhibits gastric cancer progression via the miR-224/Smad4/ADAR1 successive regulatory circuit.; Authors - Cunying Ma

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hadil (Verified Customer) (05-24-2023)

I prepared this antibody in odyssey blocker at 1:1000 and incubated at 4 C overnight and seemed to get a good signal for p21. There were some non-specific bands properly caused by long incubation periods. So, i would recommend using it at this concentration for 1.5-2 hr at RT.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mammalian cell lines

FH

Malak (Verified Customer) (04-22-2021)

The best incubation period for this antibody is 1.5 hr at room temperature, longer incubations (especially overnight at 4°C) may result in aspecific bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Hepatic cancer cell extract

FH

Susan (Verified Customer) (12-03-2019)

We had been having issues with our old P21 antibody and this one worked great.

• Applications: Western Blot,
• Cell Tissue Type: keratinocytes, dog

FH

Béatrice (Verified Customer) (09-04-2019)

This is an excellent and specific antibody, without background.When prepared in 3%BSA, it can be reused several times and kept at-20°C

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HCT116

P21 Antibody Western Blot, validation (1/1000 dilution) in HCT116 (Cat no:10355-1-AP)

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