Anticorps Recombinant de lapin anti-P21

P21 Recombinant Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

3N14

N° de cat : 82669-2-RR

Synonymes

CDKN1A, P21;CDKN1A, 3N14, CDK-interacting protein 1, CIP1

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Galerie de données de validation

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  WB analysis using 82669-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82669-2-RR (P21 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 82669-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82669-2-RR (P21 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 82669-2-RR

  IP result of anti-P21 (IP:82669-2-RR, 4ug; Detection:82669-2-RR 1:800) with MCF-7 cells lysate 760 ug.

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  IF Staining of MCF-7 using 82669-2-RR

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using P21 antibody (82669-2-RR, Clone: 3N14 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 82669-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82669-2-RR against Human P21 were performed. The affinity constant is 112 nM.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HCT 116, cellules HeLa, cellules HepG2, cellules HUVEC, cellules MCF-7, cellules MG U-87, cellules THP-1
• Résultats positifs en IP: cellules MCF-7,
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

82669-2-RR cible P21 dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: P21 Protéine recombinante Ag0368
• Nom complet: cyclin-dependent kinase inhibitor 1A (p21, Cip1)
• Masse moléculaire calculée: 18 kDa
• Poids moléculaire observé: 21 kDa
• Numéro d’acquisition GenBank: BC000275
• Symbole du gène: p21
• Identification du gène (NCBI): 1026
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CDKN1A (p21, CIP1, WAF1) is a cyclin-dependent kinase inhibitor. CDKN1A binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at the G1 phase. The expression of CDKN1A is induced by wild-type but not mutant p53 protein, through which CDKN1A mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. CDKN1A can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. CDKN1A was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported.

Publications

Species	Application	Title
	WB	Nat Commun; Foxk1 and Foxk2 promote cardiomyocyte proliferation and heart regeneration; Authors - Dongcheng Cai
human	WB	J Biol Chem; The P53-P21-RB1 pathway promotes BRD4 degradation in liver cancer through USP1; Authors - Neng Li

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