Anticorps Polyclonal de lapin anti-P2RX7

P2RX7 Polyclonal Antibody for FC, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

15

N° de cat : 11144-1-AP

Synonymes

P2RX7, P2X7

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Galerie de données de validation

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  IHC staining of human prostate cancer using 11144-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11144-1-AP (P2RX7 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human tonsillitis using 11144-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11144-1-AP (P2RX7 Antibody) at dilution of 1:400 (under 10x lens).

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  IHC staining of human tonsillitis using 11144-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11144-1-AP (P2RX7 Antibody) at dilution of 1:400 (under 40x lens).

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  IF Staining of HeLa using 11144-1-AP

  Immunofluorescent analysis of HeLa cells using 11144-1-AP (P2RX7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 11144-1-AP

  1X10^6 HeLa cells were stained with 0.2ug P2RX7 antibody (11144-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu d'amygdalite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 11 publications below
• IHC: See 2 publications below
• IF: See 4 publications below

Informations sur le produit

11144-1-AP cible P2RX7 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: P2RX7 Protéine recombinante Ag1600
• Nom complet: purinergic receptor P2X, ligand-gated ion channel, 7
• Masse moléculaire calculée: 69 kDa
• Numéro d’acquisition GenBank: BC011913
• Symbole du gène: P2RX7
• Identification du gène (NCBI): 5027
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Nucleotides, the structural subunits of the nucleic acids, are also important extracellular signaling molecules. P2 receptors are a family of cell surface receptors that mediate a wide variety of physiologic effects in response to extracellular nucleotides. These receptors fall into two classes: P2X receptors, which are ligand-gated ion channels that mediate calcium and potassium fluxes in response to ATP, and P2Y receptors, which are G protein-coupled receptors (GPCRs) (PMID: 21724990). P2RX7 functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. P2RX7 is highly expressed by cells of the haemopoietic lineage and can mediate cell death, killing of infectious organisms, and regulation of the inflammatory response (PMID: 22102807).

Publications

Species	Application	Title
human	IF	J Immunol; The danger signal, extracellular ATP, is a sensor for an airborne allergen and triggers IL-33 release and innate Th2-type responses.; Authors - Kouzaki Hideaki H
mouse	WB	Front Cell Neurosci; Clemastine Alleviates Depressive-Like Behavior Through Reversing the Imbalance of Microglia-Related Pro-inflammatory State in Mouse Hippocampus.; Authors - Wen-Jun Su
		J Leukoc Biol; HMG-CoA reductase inhibitors activate caspase-1 in human monocytes depending on ATP release and P2X7 activation.; Authors - Liao Yi-Hsiang YH
human	IF	J Exp Clin Cancer Res; Shock wave-induced ATP release from osteosarcoma U2OS cells promotes cellular uptake and cytotoxicity of methotrexate.; Authors - Baochang Qi
rat	WB,IHC	J Biochem Mol Toxicol; Blockade of P2X7 receptor-mediated purinergic signaling with A438079 protects against LPS-induced liver injury in rats; Authors - Adem Kara
mouse	WB	Toxicol Lett; Maternal smoking during pregnancy aggravated muscle phenotype in FHL1-/y offspring mice similar to congenital clubfoot through P2RX7-mediated pyroptosis.; Authors - Yi Lou

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