Anticorps Monoclonal anti-P2RY1

P2RY1 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(4)

CloneNo.

1A1F3

N° de cat : 67654-1-Ig

Synonymes

ADP receptor, ATP receptor, P2Y purinoceptor 1, P2Y1, Purinergic receptor

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Galerie de données de validation

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  WB analysis using 67654-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67654-1-Ig (P2RY1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  IHC staining of mouse brain using 67654-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67654-1-Ig (P2RY1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 67654-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67654-1-Ig (P2RY1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 67654-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67654-1-Ig (P2RY1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 67654-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67654-1-Ig (P2RY1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat brain using 67654-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using P2RY1 antibody (67654-1-Ig, Clone: 1A1F3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of rat brain using 67654-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using P2RY1 antibody (67654-1-Ig, Clone: 1A1F3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse brain using 67654-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using P2RY1 antibody (67654-1-Ig, Clone: 1A1F3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse brain using 67654-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using P2RY1 antibody (67654-1-Ig, Clone: 1A1F3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de porc, tissu cérébral de lapin, tissu cérébral de rat, tissu cérébral de souris, tissu de cervelet de porc, tissu de cervelet de rat, tissu de cervelet de souris
• Résultats positifs en IHC: tissu cérébral de souris, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de rat, tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

67654-1-Ig cible P2RY1 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: P2RY1 Protéine recombinante Ag12762
• Nom complet: purinergic receptor P2Y, G-protein coupled, 1
• Masse moléculaire calculée: 373 aa, 42 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC074785
• Symbole du gène: P2RY1
• Identification du gène (NCBI): 5028
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	IHC	Sci China Life Sci; Inflammatory macrophages exacerbate neutrophil-driven joint damage through ADP/P2Y1 signaling in rheumatoid arthritis.; Authors - Xiaoyu Zhang
human	WB	Oncol Lett; New prognostic factors and scoring system for patients with acute myeloid leukemia.; Authors - Ye Kuang
rat	WB,IF	Neuroscience; Purinergic Astrocyte Signaling Driven by TNF-α After Cannabidiol Administration Restores Normal Synaptic Remodeling Following Traumatic Brain Injury; Authors - Tenghan Ling
mouse	WB	Nat Commun; Astrocytic neuroligin 3 regulates social memory and synaptic plasticity through adenosine signaling in male mice; Authors - Rui Dang

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