Anticorps Polyclonal de lapin anti-PABPC1,PABP

• Phare
• Validé par KD/KO

PABPC1,PABP Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, RIP, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

21

Avis (3)

N° de cat : 10970-1-AP

Synonymes

PAB1, PABP, PABP 1, PABP1, PABPC1, PABPC1,PABP, PABPC2, PABPL1, Poly(A) binding protein 1

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Galerie de données de validation

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  WB analysis using 10970-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10970-1-AP (PABPC1,PABP antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10970-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10970-1-AP (PABPC1,PABP antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10970-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10970-1-AP (PABPC1,PABP antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10970-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10970-1-AP (PABPC1,PABP antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 10970-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10970-1-AP (PABPC1,PABP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 10970-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 10970-1-AP (PABPC1,PABP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10970-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10970-1-AP (PABPC1,PABP antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 10970-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10970-1-AP (PABPC1,PABP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10970-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10970-1-AP (PABPC1,PABP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10970-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10970-1-AP (PABPC1,PABP antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 10970-1-AP

  IP Result of anti-PABPC1,PABP (IP:10970-1-AP, 4ug; Detection:10970-1-AP 1:700) with mouse testis tissue lysate 4000ug.

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  IHC staining of human pancreas using 10970-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 10970-1-AP (PABPC1,PABP antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 10970-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 10970-1-AP (PABPC1,PABP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 10970-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10970-1-AP (PABPC1,PABP Antibody) at dilution of 1:50.

• 

  IHC staining of human testis using 10970-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10970-1-AP (PABPC1,PABP Antibody) at dilution of 1:50.

• 

  IF Staining of MCF-7 using 10970-1-AP

  Immunofluorescent analysis of MCF-7 cells, using PABPC1 antibody 10970-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  FC experiment of MCF-7 using 10970-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug PABPC1,PABP antibody (10970-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules HCT 116, cellules A549, cellules HeLa, cellules HT-29, cellules MCF-7, cellules NCI-H1299, cellules PC-3, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IP: tissu testiculaire de souris
• Résultats positifs en IHC: tissu pancréatique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7
• Résultats positifs en cytométrie: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 16 publications below
• IHC: See 3 publications below
• IF: See 4 publications below
• IP: See 2 publications below
• RIP: See 1 publications below

Informations sur le produit

10970-1-AP cible PABPC1,PABP dans les applications de WB, RIP, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PABPC1,PABP Protéine recombinante Ag1422
• Nom complet: poly(A) binding protein, cytoplasmic 1
• Masse moléculaire calculée: 71 kDa
• Poids moléculaire observé: 71 kDa
• Numéro d’acquisition GenBank: BC015958
• Symbole du gène: PABPC1
• Identification du gène (NCBI): 26986
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The poly(A)-binding protein (PABP), which is found complexed to the 3-prime poly(A) tail of eukaryotic mRNA, is required for poly(A) shortening and translation initiation [PMID: 21989405]. Polyadenylate-binding protein 1 (PABPC1) is a cytoplasmic-nuclear shuttling protein important for protein translation initiation, and both RNA processing and stability. In the cytoplasm, PABPC1 binds to the 3' poly(A) tail of eukaryotic mRNAs through its RNA-recognition motifs (RRM) and interacts with the N-terminus of eIF4G, part of the eIF4F complex associated with the 5' cap structure [PMID:20009508, 17381337].

Publications

Species	Application	Title
human	WB	Exp Mol Med; The deubiquitinating enzyme STAMBP is a newly discovered driver of triple-negative breast cancer progression that maintains RAI14 protein stability; Authors - Qianqian Yang
human	WB	Nat Commun; An oncopeptide regulates m6A recognition by the m6A reader IGF2BP1 and tumorigenesis.; Authors - Song Zhu
human	WB	J Exp Med; E3 ligase MKRN3 is a tumor suppressor regulating PABPC1 ubiquitination in non-small cell lung cancer.; Authors - Ke Li
human	WB	Clin Transl Med; LncRNA BC promotes lung adenocarcinoma progression by modulating IMPAD1 alternative splicing; Authors - Qi Wen Chen
rat	IHC	Leukemia; An atlas of bloodstream-accessible bone marrow proteins for site-directed therapy of acute myeloid leukemia.; Authors - L Angenendt
mouse	WB,IP	Haematologica; YTHDF3 modulates hematopoietic stem cells by recognizing RNA m6A modification on Ccnd1.; Authors - Xiaofei Zhang ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Elisa (Verified Customer) (01-13-2023)

PABPC1 (Poly(A) Binding Protein Cytoplasmic 1) staining (in magenta) and Hoechst (nuclear staining) in blue. PABPC1 ab shows a clear citoplasma staining. Method: RPE1 cells were fixed in cold methanol for 10' at -20C. Cells were then rehydrated with PBS for 5'. Membrane permeabilization was then performed with 0.1% Triton + 0.1% Tween +0.01%SDS in PBS for 10'. Cells were finally incubated with blocking buffer (5% BSA+ 0.1% Tween in PBS) for 30' at RT. Primary antibody was diluted in blocking buffer 1:200 and incubated for 1h at room temperature. Alexa-488-Anti-rabbit was used as secondary antibody (1:600 dilution) (1h at room temperature).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Human retinal pigment epithelial-1 (RPE-1)

PABPC1,PABP Antibody Immunofluorescence validation (1:200 dilution) in Human retinal pigment epithelial-1 (RPE-1) (Cat no:10970-1-AP)

FH

Zee (Verified Customer) (01-28-2020)

It worked very well when I performed western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse heart tissue

FH

George (Verified Customer) (09-11-2019)

Rb PABP Ig shows very little endogenous background staining with clear dispersed staining seen both in the nucleus and cytoplasm. 90mins heat shock at 42oC led to granular formation in the cytoplasm

• Applications: Immunohistochemistry, Other,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Spinal Cord/Cortical Slice Culture