Anticorps Polyclonal de lapin anti-PACSIN1

PACSIN1 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 13219-1-AP

Synonymes

KIAA1379, PACSIN1, SDPI

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Galerie de données de validation

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  WB analysis of mouse cerebellum using 13219-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 13219-1-AP (PACSIN1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 13219-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13219-1-AP (PACSIN1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 13219-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13219-1-AP (PACSIN1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human brain using 13219-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13219-1-AP (PACSIN1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human brain using 13219-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13219-1-AP (PACSIN1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 13219-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13219-1-AP (PACSIN1 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of SH-SY5Y using 13219-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 13219-1-AP (PACSIN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of SH-SY5Y using 13219-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 13219-1-AP (PACSIN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu de cervelet de souris, tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below

Informations sur le produit

13219-1-AP cible PACSIN1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PACSIN1 Protéine recombinante Ag4102
• Nom complet: protein kinase C and casein kinase substrate in neurons 1
• Masse moléculaire calculée: 444 aa, 51 kDa
• Poids moléculaire observé: 48-51 kDa
• Numéro d’acquisition GenBank: BC040228
• Symbole du gène: PACSIN1
• Identification du gène (NCBI): 29993
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PACSIN1 (also known as syndapin-1) is a member of the protein kinase C and casein kinase substrate in neurons (PACSIN) family. In mammals, the PACSIN family is comprised of three members, PACSIN1, PACSIN2, and PACSIN3 (PMID: 34990060). PACSIN1 is expressed mainly in neurons, whereas PACSIN2 is ubiquitously expressed in all tissues, and PACSIN3 is expressed mainly in skeletal muscle and the heart (PMID: 23668323). All of these three members contain an N-terminal F-BAR domain and a C-terminal SH3 domain. PACSIN1 plays a role in endocytosis and endosomal recycling. Meanwhile, it has a role in actin remodeling and microtubule nucleation and also plays a particular role in membrane shaping and reconstruction (PMID: 23035120; 34422904). PACSIN1 is involved in neuromorphogenesis and the regulation of the nervous system, and the inappropriate expression of PACSIN1 has been associated with some neurological diseases, including schizophrenia, Alzheimer's disease, and Huntington's disease (PMID: 34990060).

Publications

Species	Application	Title
human	IHC	Front Mol Biosci; Decreased Expression of PACSIN1 in Brain Glioma Samples Predicts Poor Prognosis.; Authors - Zhou Zimu