Anticorps Polyclonal de lapin anti-PACSIN2

• Phare
• Validé par KD/KO

PACSIN2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

7

Avis (1)

N° de cat : 10518-2-AP

Synonymes

PACSIN2, SDPII, Syndapin 2

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 10518-2-AP

  WB result of PACSIN2 antibody (10518-2-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PACSIN2 transfected NIH/3T3 cells.

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  WB analysis of NIH/3T3 using 10518-2-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10518-2-AP (PACSIN2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 10518-2-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 10518-2-AP (PACSIN2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 10518-2-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10518-2-AP (PACSIN2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 10518-2-AP

  IP Result of anti-PACSIN2 (IP:10518-2-AP, 4ug; Detection:10518-2-AP 1:500) with NIH/3T3 cells lysate 1200ug.

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  IHC staining of human breast cancer using 10518-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10518-2-AP (PACSIN2 Antibody) at dilution of 1:400 (under 10x lens).

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  IHC staining of human breast cancer using 10518-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10518-2-AP (PACSIN2 Antibody) at dilution of 1:400 (under 40x lens).

• 

  IHC staining of human breast cancer using 10518-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10518-2-AP (PACSIN2 antibody) at dilution of 1:100 (under 25x lens).

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  IHC staining of human skin using 10518-2-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 10518-2-AP (PACSIN2 Antibody) at dilution of 1:400 (under 10x lens).

• 

  IHC staining of human skin using 10518-2-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 10518-2-AP (PACSIN2 Antibody) at dilution of 1:400 (under 40x lens).

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  IF Staining of NIH/3T3 using 10518-2-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed NIH/3T3 cells using 10518-2-AP (PACSIN2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, tissu cardiaque de souris, tissu pulmonaire de souris
• Résultats positifs en IP: cellules NIH/3T3
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu cutané humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IF: See 4 publications below

Informations sur le produit

10518-2-AP cible PACSIN2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PACSIN2 Protéine recombinante Ag0809
• Nom complet: protein kinase C and casein kinase substrate in neurons 2
• Masse moléculaire calculée: 56 kDa
• Poids moléculaire observé: 60-65 kDa
• Numéro d’acquisition GenBank: BC008037
• Symbole du gène: PACSIN2
• Identification du gène (NCBI): 11252
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Peripheral membrane proteins of the Bin/amphiphysin/Rvs (BAR) and Fer-CIP4 homology-BAR (F-BAR) family participate in cellular membrane trafficking (PMID: 19549836). PACSIN2 (also known as syndapin-2) is a member of the protein kinase C and casein kinase substrate in neurons (PACSIN) family, which are membrane-binding proteins characterized by an amino-terminal F-BAR domain. PACSIN2 plays a role in intracellular vesicle-mediated transport. It is involved in the endocytosis of cell-surface receptors like the EGF receptor, contributing to its internalization (PMID: 23129763). PACSIN2 also has a role in caveolae membrane sculpting (PMID: 21610094).

Publications

Species	Application	Title
human	WB,IF	Mol Biol Cell; Cooperation of MICAL-L1, syndapin2, and phosphatidic acid in tubular recycling endosome biogenesis.; Authors - Giridharan Sai Srinivas Panapakkam SS ; KD Validated
human	WB	PLoS One; Role of Phosphatidylinositol 4,5-Bisphosphate in Regulating EHD2 Plasma Membrane Localization.; Authors - Simone Laura C LC ; KD Validated
human	WB,IF	J Bacteriol; Application of a C. trachomatis expression system to identify C. pneumoniae proteins translocated into host cells.; Authors - Izumi Yanatori
human	WB	Cell Rep; Endogenous Cyclin D1 Promotes the Rate of Onset and Magnitude of Mitogenic Signaling via Akt1 Ser473 Phosphorylation.; Authors - Ke Chen
human	WB	J Dent Res; Proteome Analysis of Temporomandibular Joint with Disc Displacement; Authors - X Liu
mouse	WB,IF	Nat Commun; The molecular organization of differentially curved caveolae indicates bendable structural units at the plasma membrane; Authors - Claudia Matthaeus

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Herve (Verified Customer) (11-13-2019)

This antibody recognizes a 55-kDa band that is present in both Pacsin2 WT and KO platelet lysates. PACSIN2 runs normally at 65 kDa in human and mouse platelet lysates and is absent in mouse Pacsin2 KO samples.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Platelets